FIGURE

Figure 3

ID

ZDB-FIG-230131-20

Publication

Sieliwonczyk et al., 2023 - Improved selection of zebrafish CRISPR editing by early next-generation sequencing based genotyping

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Figure 3

Effect of distance to the cut site on KI efficiency. (A) Frequency of KI per embryo for the non-target asymmetric PAM distal ssODN conformation for the LQTS locus (n = 37). (B) Frequency of KI per embryo for the target asymmetric PAM proximal ssODN conformation for the LQTS locus (n = 53). (C) Frequency of KI per embryo for the non-target asymmetric PAM distal ssODN conformation for the BrS locus (n = 82). (D) Frequency of KI per embryo for the target asymmetric PAM proximal ssODN conformation for the BrS locus (n = 44). Statistical testing was performed with negative binomial regression with use of the Tukey’s HSD (honestly significant difference) test for multiple correction, not significant results are not displayed, ***p-value < 0.001, **p-value < 0.01, *p-value < 0.05. KI knock-in, NAD non-target asymmetric PAM distal, TAP target asymmetric PAM proximal.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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