Fig. 6

Administration of oxytocin analogue, dexamethasone and aspirin restores immune response and social behavior in zebrafish. a Experimental set-up and drugs exposure to 1-day-old embryos. Zebrafish larvea are performed with different concentration drugs (carbetocin, dexamethasone, or aspirin), respectively. qRT-PCR analysis of the relative transcription level of IL-1β at 2 dpf upon three groups. b Carbetocin, DXMS, and aspirin markedly decreases expression of pro-inflammatory cytokines IL-1β, Ctl, egg-water administration, Carbe 0.5 μM, Carbetocin 0.5 μM treatment; Carbe 1 μM, Carbetocin 1 μM treatment; DXMS 1 μM, dexamethasone 1 μM treatment; Aspirin 1 μM, Aspirin 1 μM treatment; Aspirin 10 μM, Aspirin 10 μM treatment. +/+: scgn^+/+, +/−: scgn^+/−, −/−: scgn^−/−. DXMS Dexamethasone, a synthetic corticosteroid used to treat inflammation, Aspirin also known as acetylsalicylic acid. P-values were calculated using one-way ANOVA, Tukey’s multiple comparisons test. ****p < 0.0001. Ns, not significant. c Six-day-old embryos were exposed to drugs for 2 days, respectively. IL-6 level and social behavior tests were performed on 8 dpf. d Carbetocin, DXMS and aspirin markedly decreases expression of IL-1β. P-values were calculated using one-way ANOVA, Tukey’s multiple comparisons test. ****p < 0.0001. Ns not significant. e Contact frequency in larvea are rescued with Carbetocin and aspirin administration. +/+: scgn^+/+, −/−: scgn^−/−. P-values were calculated using one-way ANOVA, Tukey’s multiple comparisons test. ****p < 0.0001. *p < 0.05. f Duration of larvae body contact can be rescued with Carbetocin and aspirin administration. +/+: scgn^+/+, −/−: scgn^−/−. P-values were calculated using one-way ANOVA, Tukey’s multiple comparisons test. ****p < 0.0001.*p < 0.05