FIGURE

Fig. 3

ID
ZDB-FIG-220926-40
Publication
Yao et al., 2022 - Skeletal Class III Malocclusion Is Associated with ADAMTS2 Variants and Reduced Expression in a Familial Case
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Fig. 3

Phenotype in adamts2-knockdown zebrafish. (A) Zebrafish larvae were imaged in lateral positions with transmitted light at 48 hpf, 72 hpf, and 96 hpf. (B,C) Schematic diagram of zebrafish craniofacial structure from ventral view and lateral view: (bh) basihyal; (ch) ceratohyal; (co) coracoids of pectoral girdle; (cb) ceratobranchial; (c) cleithrum; (ep) ethmoid plate; (hs) hyosymplectic; (m) Meckel’s cartilage; (oa) occipital arch; (pp) pterygoid process of the palatoquadrate; (pq) palatoquadrate. (D,E) Control and (F,G) adamts2-knockdown zebrafish at 144 hpf were stained with Alcian blue to observe craniofacial cartilage structures from ventral and dorsal view. The red double arrow represents the measurement method of related indicators. (H) Ethmoid plate length and width; (I) palatoquadrate length; (J) Meckel’s cartilage length and width. (K) Scatter histogram showing change of palatoquadrate length, as well as length and width with respect to Meckel’s cartilage and ethmoid plate in control and adamts2-knockdown zebrafish. (L) Comparison of the sox3 protein expression levels in control crispant and adamts2 crispant zebrafish at 72 hpf and 96 hpf. n = 3 independent replicates for all experiments. For (DK): control crispant n = 56 embryos, adamts2 crispant n = 41 embryos. Error bar represents the SD. ns, no significance, *** p < 0.001. Scale bars: 500 μm (A), 100 μm (DJ).

Expression Data
Gene:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage Range: Protruding-mouth to Day 4

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Protruding-mouth to Day 6

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.