FIGURE

Fig. 4

ID
ZDB-FIG-220923-81
Publication
Williams et al., 2022 - Assessment of endocytic traffic and Ocrl function in the developing zebrafish neuroepithelium
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Fig. 4

Ocrl localises to early endocytic compartments in neuroepithelial cells. (A) Representative confocal images of live 28 hpf zebrafish embryos transiently expressing EGFP–Ocrla after hindbrain ventricle injection of RAP–Cy3 or DQ-BSA. Regions within the dashed boxes are magnified in the lower panels. Arrowheads point to co-localisation between Ocrla signal and RAP–Cy3 or DQ-BSA. Scale bars: 5 µm. (B) Representative images of fixed tissue sections through the hindbrain of embryos transiently expressing EGFP–Ocrla and stained with antibodies against EEA1, Lrp2, Golgin-84 or LAMP1. Arrowheads indicate instances of co-localisation between Ocrla and the indicated marker. Scale bars: 5 µm. (C) Quantification of co-localisation between Ocrla puncta and EEA1 (n=3), Lrp2 (n=10), Golgin-84 (n=4) or LAMP1 (n=4) on fixed tissue sections. Each data point represents one individual embryo. Error bars represent the s.d.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.