Fig. 1

FIG. 1. Different treatments affect social interaction and color preference in zebrafish. (A) Schematic describing the experimental procedure for Ctrl, OTC, and LGG (3-week treatment), or Ctrl and OTC+LGG (6-week treatment). The behavioral trajectory (B) and the mean velocity (C) of the zebrafish from control group (Ctrl) and exposure groups (OTC, LGG, and OTC+LGG) were tested during 10-min period (n = 12/group, four groups per treatment). (D) Schematic describing the testing index of social interaction. (E) The shoaling behavior of zebrafish larvae through OTC, LGG, and OTC+LGG treatment (n = 4/group, 24 groups per treatment). (F) The shoals of zebrafish exhibit the color preference with six color combinations (B-R, B-Y, B-G, Y-R, Y-G, G-R) for OTC, LGG, and OTC+LGG treatment. The percentage of numbers of 20 larval zebrafish spent in each colored zone was counted every 20 s for a total of 10 min (n = 10 groups for Ctrl [3 weeks], n = 8 groups for OTC [3 weeks], n = 9 groups for LGG [3 weeks], n = 8 groups for Ctrl [6 weeks], n = 8 groups for OTC+LGG [6 weeks]). *p < 0.01, **p < 0.01, ***p < 0.001, ns, not significant. OTC, oxytetracycline; LGG, Lactobacillus rhamnosus GG.