Figure 6

nlrc3-like deficiency leads to excessive neutrophils infiltration and tissue damage at later stages of infection. (A) SB staining of the tail region in different groups of zebrafish embryos. (B) H&E staining of paraffin sections of the caudal vein plexus. (C) Quantification of the number of infiltrated immune cells in (B, D) Acid fast staining of paraffin sections in the caudal vein plexus. (E) Statistics of normalized bacterial fluorescence intensity at late stage of infection. (F) Quantitative RT-PCR assessed the expression of multiple cytokines at a late stage of infection (6 dpi). The values are averages from three independent biological repeats, 25 embryos per group in each repeat. (G) Survival curves of infected zebrafish with DEX treatment. The data is from one of two similar independent biological replicates (H). SB staining showing neutrophil recruitment to infection sites at 5 hpi. The left panel shows the whole embryo while the right panel shows an enlargement of the region in the red box that was used for counting neutrophils. (I) Graph showing the number of neutrophils, counted as in (H). The data shown was combined from three independent biological repeats. (A, B, D, H) Scale bar = 50 μm *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ns, not statistically significant (p>0.05).