FIGURE

Figure 5

ID

ZDB-FIG-220616-22

Publication

Niu et al., 2022 - Negative Regulator Nlrc3-like Maintain the Balanced Innate Immune Response During Mycobacterial Infection in Zebrafish

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Figure 5

nlrc3-like deficiency promotes neutrophil recruitment. (A) Survival curves of nlrc3-like -/- and non-mutant embryos after M. marinum infection, based on results from two independent biological replicates. (B) Numbers of neutrophils recruited to infection sites at the indicated time points. (C) Live imaging of neutrophils after M. marinum infection. 3 dpf Tg (lyz-GFP) zebrafish embryos were infected with M. marinum:Tdtomato. Time interval, 1 min 30 s. The data is representative of three independent biological replicates. (D) SB staining showing neutrophil recruitment to infection sites. The left panel shows the whole embryo while the right panel shows magnification of the area in the red box used for counting neutrophils. (E) Graph of neutrophils counted in (D, F) Graph of the neutrophils counted after SB staining of β-actin-nlrc3l (whole body expression of nlrc3-like) and β-actin-6Xmyc embryos. (G) Graph of neutrophils counted after SB staining in coro1a-nlrc3l (myeloid expression of nlrc3-like) and coro1a-6Xmyc embryos (C, D). Scale bar = 50 μm *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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