Figure 5

M. marinum infection impacts on macrophages viability in the regenerated caudal fin. (A) Expression of tnfa transcripts in uncut and cut caudal fins after injection of PBS (CT) or infection with LD, MD or HD of M. marinum at 3 hpA. Analysis was performed by RT-qPCR and results are expressed as the tnfa/ef1a ratio (mean ± SEM, n=5-6, Kruskal-Wallis, Dunn’s multiple comparisons test, *p ≤ 0.05, **p ≤ 0.01). (B) Expression of il1b transcripts in uncut and cut caudal fins after injection of PBS (CT) or infection with LD, MD or HD of M. marinum at 3 hpA. Analysis was performed by RT-qPCR and results are expressed as the il1b/ef1a ratio (mean ± SEM, n = 5-6, Kruskal-Wallis, Dunn’s multiple comparisons test, *p≤ 0.05). (C) Experimental design to study macrophage viability using flow cytometry. (D) Percentage of total apoptotic and necrotic macrophages (mpeg⁺ cells) in the whole larva after injection of PBS (CT) or infection with LD, MD or HD of M. marinum at 48 hpA was obtained by flow cytometry (mean ± SEM, n = 4-5, Mann Whitney test, one tailed, *p ≤ 0.05).