PUBLICATION
Exploring Macrophage-Dependent Wound Regeneration During Mycobacterial Infection in Zebrafish
- Authors
- Bohaud, C., Johansen, M.D., Varga, B., Contreras-Lopez, R., Barthelaix, A., Hamela, C., Sapède, D., Cloitre, T., Gergely, C., Jorgensen, C., Kremer, L., Djouad, F.
- ID
- ZDB-PUB-220412-11
- Date
- 2022
- Source
- Frontiers in immunology 13: 838425 (Journal)
- Registered Authors
- Djouad, Farida
- Keywords
- Mycobacterium marinum, infection, macrophages sub-types, necrosis, regeneration, zebrafish
- MeSH Terms
-
- Animals
- Macrophages/metabolism
- Mycobacterium Infections*
- Mycobacterium marinum*
- Zebrafish/metabolism
- Zebrafish Proteins/metabolism
- PubMed
- 35401552 Full text @ Front Immunol
Citation
Bohaud, C., Johansen, M.D., Varga, B., Contreras-Lopez, R., Barthelaix, A., Hamela, C., Sapède, D., Cloitre, T., Gergely, C., Jorgensen, C., Kremer, L., Djouad, F. (2022) Exploring Macrophage-Dependent Wound Regeneration During Mycobacterial Infection in Zebrafish. Frontiers in immunology. 13:838425.
Abstract
The molecular and cellular mechanisms associated with tissue degradation or regeneration in an infectious context are poorly defined. Herein, we explored the role of macrophages in orchestrating either tissue regeneration or degradation in zebrafish embryos pre-infected with the fish pathogen Mycobacterium marinum. Zebrafish were inoculated with different infectious doses of M. marinum prior to fin resection. While mild infection accelerated fin regeneration, moderate or severe infection delayed this process by reducing blastemal cell proliferation and impeding tissue morphogenesis. This was correlated with impaired macrophage recruitment at the wound of the larvae receiving high infectious doses. Macrophage activation characterized, in part, by a high expression level of tnfa was exacerbated in severely infected fish during the early phase of the regeneration process, leading to macrophage necrosis and their complete absence in the later phase. Our results demonstrate how a mycobacterial infection influences the macrophage response and tissue regenerative processes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping