FIGURE

Fig. 5

ID

ZDB-FIG-220209-5

Publication

Janiak et al., 2022 - Non-telecentric two-photon microscopy for 3D random access mesoscale imaging

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Fig. 5

Mesoscale imaging of zebrafish larvae.

a Photograph of two 9 dpf zebrafish larvae mounted head-to-head in a microscope chamber with mm-scale ruler in background. b The same 2 fish (HuC:GCaMP6f) as in (a) imaged under 2-photon with nTC₂ 3.5 mm FOV configuration, at 512 × 128 px (3.91 Hz). c, d Activity-correlation (cf. Fig. 3c) of the scan in (b) during presentation of full-field flashes of ultraviolet (UV)-light, with hand-selected exemplary ROIs, extracted time-traces (d) and light-stimulus-aligned averages (e). f–i The same fish as shown on the left in (b, fish 1), now shown at full 3.5 mm field of view (f, 512 × 128 px, 3.91 Hz) and increased spatial resolution scans of regions as indicated to reveal cellular detail (g–i, 1024 × 1024 px, 0.49 Hz). RGC, retinal ganglion cell.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.