FIGURE

Figure 1

ID

ZDB-FIG-211201-172

Publication

Chen et al., 2021 - Knockout of mafba Causes Inner-Ear Developmental Defects in Zebrafish via the Impairment of Proliferation and Differentiation of Ionocyte Progenitor Cells

Other Figures

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Figure 1

CRISPR/Cas9-mediated mutagenesis of mafba. (A) The sole exon of two mafba transcripts and the CRISPR/Cas9 target site are shown. The 5′UTR, CDS, and 3′UTR regions of the two mafba transcripts are shown in detail. (B) DNA sequencing identifies the c.298_304delACTCCTA mafba mutation lines. The 7-bp deletion is indicated with a black frame. (C) Relative expressions of mafba determined by qRT-PCR analysis in sibling, mafba^+/−, and mafba^−/− embryos at 4 dpf. The β-actin served as the endogenous control. Data are represented as mean ± SD; *, p < 0.05; **, p < 0.01. (D) Western blot analysis shows the similar protein expression of Mafba between siblings and mafba^+/− embryos, but a significant decrease in mafba^−/− mutants at 4 dpf. GAPDH was used as the internal control.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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