Figure 2

A representative frozen section (× 200, hematoxylin and eosin) (a) of a liver was obtained from zebrafish No. 5 (low fatty liver group) and stained with oil red O. In the same area of Fig. 1a, a 10% fatty change was observed with oil red O positive stain (box) (× 200) (b). Higher magnification of the oil red O positive area (lower box marked with solid line in Fig. 1b) (× 400) (c). A representative section of oil red O stain from the zebrafish before feeding a high cholesterol diet (× 200) (d) (see the workflow diagram of Fig. 1). The high fatty liver group contains zebrafish that have a proportion score of no less than 50 (× 200) (e). At higher magnification (yellow box in Fig. 1e), both macrovesicular and microvesicular fatty changes were detected at high levels in the high fatty liver group compared to the negative internal control of the glandular epithelial cells in the stomach (left upper side) (× 400) (f).