PUBLICATION
Long noncoding RNAs associated with nonalcoholic fatty liver disease in a high cholesterol diet adult zebrafish model
- Authors
- An, H.J., Lee, Y.J., Choe, C.P., Cho, H.K., Song, D.H.
- ID
- ZDB-PUB-211129-75
- Date
- 2021
- Source
- Scientific Reports 11: 23005 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Cholesterol, Dietary/adverse effects*
- Disease Models, Animal
- Humans
- Liver/metabolism
- Liver/pathology
- Non-alcoholic Fatty Liver Disease/etiology
- Non-alcoholic Fatty Liver Disease/genetics*
- Non-alcoholic Fatty Liver Disease/pathology
- RNA, Long Noncoding/genetics*
- Transcriptome
- Zebrafish/genetics
- PubMed
- 34837012 Full text @ Sci. Rep.
Citation
An, H.J., Lee, Y.J., Choe, C.P., Cho, H.K., Song, D.H. (2021) Long noncoding RNAs associated with nonalcoholic fatty liver disease in a high cholesterol diet adult zebrafish model. Scientific Reports. 11:23005.
Abstract
The mechanism of nonalcoholic fatty liver disease (NAFLD) has not been completely revealed. In this study, we investigated the association of liver histological changes and long noncoding RNAs (lncRNAs) in the NAFLD zebrafish model. Forty zebrafish were fed a high-cholesterol diet (1.5 g per day) for 8 weeks. We measured fatty liver changes in the zebrafish liver using oil red O staining and divided them into two groups based on high and low scores. We pooled each group of zebrafish livers and identified lncRNAs, miRNAs, and mRNAs using Next-generation sequencing. Human homologs of lncRNAs were identified using ZFLNC, Ensembl, and NONCODE. We found several significant genes, including 32 lncRNAs, 5 miRNA genes, and 8 protein-coding genes, that were associated with liver metabolism and NAFLD-related functions in zebrafish. In particular, eight conserved human homologs of lncRNAs were found. We discovered the human homologs of eight lncRNA candidates from fatty liver zebrafish for the first time. The spectrum of biological mechanisms by which lncRNAs mediate their functional roles in NAFLD in a high cholesterol diet adult zebrafish model remains to be uncovered.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping