kif2a−/− larvae exhibit locomotor abnormalities, increased locomotor behavior to PTZ and habituation learning disabilities. a, Average total movement of kif2a+/+ and kif2a−/− larvae from 5 to 8 dpf expressed in actinteg units. Values are reported as the mean ± SD of four separate experiments. Significant values are noted as ****p ≤ 0.0001, ***p ≤ 0.001, **p ≤ 0.01, and *p ≤ 0.05. b, Average total movement of 5 dpf kif2a+/+ and kif2a−/− larvae after 6 mm PTZ immersion for 30 min. Values are reported as the mean ± SD of four separate experiments. Significant values are noted as ***p ≤ 0.001. c, The habituation learning assay performed on 6 dpf kif2a+/+ and kif2a−/− larvae was composed of different protocols: starting with a 3 h incubation period in the light (indicated in yellow), followed by a habituation training consisting of four periods with 120 DFs with a 15 s ISI (regions with gray and yellow stripes), alternated by 10 min of light, a period of 30 min pause in the light preceding the actual test, and a test consisting of 10 DFs with a 60 s ISI (region with gray and yellow stripes). d, Percentage habituation of kif2a+/+ and kif2a−/− larvae to DFs during the spaced training. Values are reported as the mean ± SD of three separate experiments. e, Habituation ratio of average movement of kif2a+/+ and kif2a−/− larvae to DFs during the test. Values are reported as the mean ± SD of three separate experiments. Significant values are noted as **p ≤ 0.01. No locomotor abnormalities and habituation learning disabilities were observed in kif2a−/− zebrafish larvae from +/– incrosses (Extended Data Fig. 3-1).
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