FIGURE

Figure 1

ID

ZDB-FIG-210902-282

Publication

Molagoda et al., 2021 - Fisetin Inhibits NLRP3 Inflammasome by Suppressing TLR4/MD2-Mediated Mitochondrial ROS Production

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Figure 1

High concentrations of fisetin are cytotoxic to BV2 microglial cells. (A) Chemical structure of fisetin. BV2 microglial cells were treated with the indicated concentrations of fisetin (0–20 µM) for 2 h in the presence or absence of 1 µg/mL LPS for 2 h, followed by treatment with 1 mM ATP (LPS/ATP) for 24 h. (B) Cell viability was analyzed using an MTT assay. (C) Images of the cells were captured using phase-contrast microscopy (×10). (D) The population of dead and viable cells (%) was measured using a Muse Cell Count & Viability Kit. (E,F) Graphical representation of the population of (E) dead and (F) viable cells. The results indicate the mean ± standard error median (SEM), and is representative of the results obtained from three independent experiments. ^# p < 0.05 vs. untreated cells; *** p < 0.001, ** p < 0.01, and * p < 0.05 vs. LPS/ATP-treated cells.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Antioxidants (Basel)