FIGURE

Figure 1

ID

ZDB-FIG-210130-16

Publication

Brunal et al., 2021 - Effects of Constitutive and Acute Connexin 36 Deficiency on Brain-Wide Susceptibility to PTZ-Induced Neuronal Hyperactivity

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Figure 1

Whole-brain activity map showing significant regional differences in neuronal activity following various PTZ concentration exposure in wild-type and cx35.5-/- zebrafish larvae. (A) Schematic describing the data collection and analysis process for whole-brain activity mapping using the MAP-mapping technique. (B) Representative atlas depicting dorsal and lateral views of the 4 major brain divisions in zebrafish, showing the telencephalon (Te) in cyan, the diencephalon (Di) in red, the mesencephalon (Me) in green, and the rhombencephalon (Rh) in magenta. (C) Representative atlas depicting smaller brain regions mentioned in the text. The olfactory bulb (OLF) is labeled in blue, the pallium (PM) in green, the subpallium (SPM) in magenta, the hypothalamus (rostral, intermediate, and caudal) in cyan (HYT), the cerebellum (CB) in red, and the inferior olive (IO) in yellow. (D–G) Colors indicate ROIs with increased (green) or decreased (magenta) pERK/tERK ratio in wild-type PTZ treated groups compared to wild-type media only group. (D) 2 mM PTZ treated (n = 10) (E) 5 mM PTZ treated (n = 8) (F) 10 mM PTZ treated (n = 10) and (G) 20 mM PTZ treated (n = 10) vs. media (n = 10). (H–K) Colors indicate ROIs with increased (green) or decreased (magenta) pERK/tERK ratio in cx35.5-/- larvae PTZ treated groups compared to cx35.5-/- media only group. (H) 2 mM PTZ treated (n = 9) (I) 5 mM PTZ treated (n = 10) (J) 10 mM PTZ treated (n = 11) and (K) 20 mM PTZ treated (n = 10) vs. media (n = 9). (L–P) Colors indicate ROIs with increased (green) or decreased (magenta) pERK/tERK ratio in cx35.5-/- groups compared to corresponding wild-type groups. (L) Media treated (WT n = 10, MUT n = 9) (M) 2 mM PTZ treated (WT n = 10, MUT n = 9) (N) 5 mM PTZ treated (WT n = 8, MUT = 10) (O) 10 mM PTZ treated (WT n = 10, MUT n = 11) and (P) 20 mM PTZ treated (WT n = 10, MUT n = 10).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mitfa^w2/w2 mitfa^w2/w2; gjd2a^fh437/fh437
Condition:	chemical treatment by environment: pentetrazol
Observed In:	cerebellum neuronal action potential dorsal telencephalon neuronal action potential hindbrain neuronal action potential hypothalamus neuronal action potential inferior olive neuronal action potential Mauthner neuron neuronal action potential olfactory bulb neuronal action potential preoptic area neuronal action potential pretectum neuronal action potential reticulospinal neuron neuronal action potential telencephalon neuronal action potential ventral telencephalon neuronal action potential vestibulospinal neuron neuronal action potential
Stage:	Day 6

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Mol. Neurosci.