FIGURE

Figure 4

ID

ZDB-FIG-201214-14

Publication

Chang et al., 2020 - Phorbol 12-Myristate 13-Acetate Induced Toxicity Study and the Role of Tangeretin in Abrogating HIF-1α-NF-κB Crosstalk In Vitro and In Vivo

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Figure 4

PMA induced elevated reactive oxygen species (ROS) and inflammatory response in Zebrafish larvae. (A) H₂ DCFDA staining on Zebrafish larvae after PMA treatment on 6 dpf larvae, scale bar = 200 μM at 4× magnification (B) Mean fluorescence intensity of H₂ DCFDA, (C) Relative mRNA expression of TNF-α, (D) Relative mRNA expression of IL-6, (E) Relative mRNA expression of IL-1β, (F) Myeloperoxidase assay, (G) Malondialdehyde assay. The data are represented as mean ± S.D. of three independent experiments * p < 0.05, ** p < 0.01, *** p < 0.001 and ns (non-significant). Control vs. PMA. Statistical significance analysis was carried out through a one-way analysis of variance (ANOVA) prism.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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