FIGURE

Figure 5.

ID

ZDB-FIG-200904-22

Publication

Hu et al., 2020 - Loss of the RNA-binding protein Rbm15 disrupts liver maturation in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Figure 5.

Inhibiting the mTORC1 pathway partially rescues the liver maturation defects in rbm15^cq96.A, confocal images (3D) showing the antibody staining of p-4Ebp1 and 2F11 to evaluate the activity of mTORC1 signal in hepatocytes at 5 dpf. B, rapamycin treatment and confocal images reflecting the rescue efficiency of rapamycin treatment. C, quantification of Dendra2 fluorescent intensity in WT, cq96, and rapamycin treatment. D, antibody staining results of Hnf4a and Dendra2 showing that rapamycin treatment affects the cellular and protein levels of Dnedra2 and Hnf4a. E, the quantification of Hnf4a fluorescent intensity in WT, cq96, and rapamycin treatment. F, WISH images showing the expression patterns of cp and gc after rapamycin treatment. rapa, rapamycin. The numbers indicate the proportion of larvae exhibiting the expression shown. Asterisks indicate statistical significance: ****, p < 0.0001. Scale bars, 100 μm; error bars, S.D.

Expression Data

Genes:	cp Dendra2 gc hnf4a
Antibodies:	Ab-2F11 Ab1-eif4ebp1 ab1-hnf4a
Fish:	WT rbm15^cq96/cq96 cq1Tg rbm15^cq96/cq96; cq1Tg
Condition:	chemical treatment by environment: sirolimus
Anatomical Terms:	hepatocyte liver
Stage Range:	Day 5 to Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	rbm15^cq96/cq96 rbm15^cq96/cq96; cq1Tg
Condition:	chemical treatment by environment: sirolimus
Observed In:	liver
Stage Range:	Day 5 to Days 7-13

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Biol. Chem.