FIGURE

Fig. 6

ID

ZDB-FIG-200724-8

Publication

Arias-Alpizar et al., 2020 - Light-triggered switching of liposome surface charge directs delivery of membrane impermeable payloads in vivo

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Fig. 6

Effect of light dose on the in vivo fate of DOPC:4 liposomes.

a Reducing the UV light dose applied to the embryo increases the time taken for DOPC:4 liposomes to transition to DOPC:3 liposomes. This, in turn, increases the resident time spent by DOPC:4 → 3 liposomes at an intermediate cationic surface charge density leading to irreversible clearance of liposomes by blood resident macrophages. b–i Whole embryo and tissue level views of DOPC:4 liposome biodistribution in kdrl:GFP embryos following embryo irradiation with variable light doses (15 min, 370 ± 7 nm, ~90 mW cm⁻², UV duty cycle stated for each image). Apparent liposome uptake in blood resident macrophages highlighted with white arrowheads. j–l Tissue level and zoomed views of DOPC:4 → 3 liposome biodistribution in mpeg1:GFP embryos following embryo irradiation at 25% UV duty cycle (15 min, 370 ± 7 nm, 0.6 J per embryo). Liposomes contained 1 mol% fluorescent lipid probe, DOPE-LR, for visualisation. Scale bars: 200 μm (whole embryo); 50 μm (tissue level), 10 μm (zoomed).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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