PUBLICATION
Light-triggered switching of liposome surface charge directs delivery of membrane impermeable payloads in vivo
- Authors
- Arias-Alpizar, G., Kong, L., Vlieg, R.C., Rabe, A., Papadopoulou, P., Meijer, M.S., Bonnet, S., Vogel, S., van Noort, J., Kros, A., Campbell, F.
- ID
- ZDB-PUB-200721-2
- Date
- 2020
- Source
- Nature communications 11: 3638 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Cations/metabolism
- Drug Delivery Systems/methods*
- Liposomes/chemistry*
- Liposomes/pharmacology
- Liposomes/therapeutic use
- Macrophages
- Membranes/metabolism
- Nanomedicine/methods
- Nanoparticles/chemistry*
- Nanoparticles/therapeutic use
- Phagocytosis
- Zebrafish
- PubMed
- 32686667 Full text @ Nat. Commun.
Citation
Arias-Alpizar, G., Kong, L., Vlieg, R.C., Rabe, A., Papadopoulou, P., Meijer, M.S., Bonnet, S., Vogel, S., van Noort, J., Kros, A., Campbell, F. (2020) Light-triggered switching of liposome surface charge directs delivery of membrane impermeable payloads in vivo. Nature communications. 11:3638.
Abstract
Surface charge plays a fundamental role in determining the fate of a nanoparticle, and any encapsulated contents, in vivo. Herein, we describe, and visualise in real time, light-triggered switching of liposome surface charge, from neutral to cationic, in situ and in vivo (embryonic zebrafish). Prior to light activation, intravenously administered liposomes, composed of just two lipid reagents, freely circulate and successfully evade innate immune cells present in the fish. Upon in situ irradiation and surface charge switching, however, liposomes rapidly adsorb to, and are taken up by, endothelial cells and/or are phagocytosed by blood resident macrophages. Coupling complete external control of nanoparticle targeting together with the intracellular delivery of encapsulated (and membrane impermeable) cargos, these compositionally simple liposomes are proof that advanced nanoparticle function in vivo does not require increased design complexity but rather a thorough understanding of the fundamental nano-bio interactions involved.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping