ZFIN ID: ZDB-FIG-200722-9
Schlegel et al., 2019 - A New Zebrafish Model for CACNA2D4-Dysfunction. Investigative ophthalmology & visual science   60:5124-5135 Full text @ Invest. Ophthalmol. Vis. Sci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: Day 5 to Adult
PHENOTYPE:
Fish:
Observed In:
Stage Range: Day 5 to Adult

Fig. 2 Cacna1fa localization cacna2d4-KO lines. (A) Immunohistochemical analysis of Cacna1fa with Zpr-1 counterstain (red-green double cones). Cacna1fa expression is not affected in cacna2d4a-KO larvae at 5 and 20 dpf, but severely reduced in cacna2d4b-KO as well as in double-KO. In the latter two, ectopic punctate Cacna1fa staining is observed distal to the OPL (arrowheads). (B) Expression in adult double-KO is comparable to WT levels, yet ectopic puncta are visible (arrowheads). Scale bars (A, B) correspond to 10 μm and apply to all images of the respective developmental stage.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cacna1fa Ab1-cacna1fa TU standard conditions Day 5 retinal outer plexiform layer IHC
Ab1-cacna1fa Days 14-20 retinal outer plexiform layer IHC
Ab1-cacna1fa Adult retinal outer plexiform layer IHC
Ab1-cacna1fa cacna2d4azh5/zh5(TU) standard conditions Day 5 retinal outer plexiform layer IHC
Ab1-cacna1fa Days 14-20 retinal outer plexiform layer IHC
Ab1-cacna1fa cacna2d4azh5/zh5; cacna2d4bzh6/zh6(TU) standard conditions Day 5 retinal outer plexiform layer IHC
Ab1-cacna1fa Day 5 retinal photoreceptor layer IHC
Ab1-cacna1fa Days 14-20 retinal outer plexiform layer IHC
Ab1-cacna1fa Days 14-20 retinal photoreceptor layer IHC
Ab1-cacna1fa Adult retinal outer plexiform layer IHC
Ab1-cacna1fa Adult retinal photoreceptor layer IHC
Ab1-cacna1fa cacna2d4bzh6/zh6(TU) standard conditions Day 5 retinal outer plexiform layer IHC
Ab1-cacna1fa Day 5 retinal photoreceptor layer IHC
Ab1-cacna1fa Days 14-20 retinal outer plexiform layer IHC
Ab1-cacna1fa Days 14-20 retinal photoreceptor layer IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
zpr-1 IHC TU standard conditions Day 5 retinal photoreceptor layer retinal cone cell
IHC Days 14-20 retinal photoreceptor layer retinal cone cell
IHC Adult retinal photoreceptor layer retinal cone cell
IHC cacna2d4azh5/zh5(TU) standard conditions Day 5 retinal photoreceptor layer retinal cone cell
IHC Days 14-20 retinal photoreceptor layer retinal cone cell
IHC cacna2d4azh5/zh5; cacna2d4bzh6/zh6(TU) standard conditions Day 5 retinal photoreceptor layer retinal cone cell
IHC Days 14-20 retinal photoreceptor layer retinal cone cell
IHC Adult retinal photoreceptor layer retinal cone cell
IHC cacna2d4bzh6/zh6(TU) standard conditions Day 5 retinal photoreceptor layer retinal cone cell
IHC Days 14-20 retinal photoreceptor layer retinal cone cell
Phenotype Details
Fish Conditions Stage Phenotype
cacna2d4azh5/zh5; cacna2d4bzh6/zh6(TU) standard conditions Day 5 retinal outer plexiform layer cacna1fa expression decreased amount, abnormal
Day 5 retinal photoreceptor layer cacna1fa expression mislocalised, abnormal
Days 14-20 retinal outer plexiform layer cacna1fa expression decreased amount, abnormal
Days 14-20 retinal photoreceptor layer cacna1fa expression mislocalised, abnormal
Adult retinal photoreceptor layer cacna1fa expression mislocalised, abnormal
cacna2d4bzh6/zh6(TU) standard conditions Day 5 retinal outer plexiform layer cacna1fa expression decreased amount, abnormal
Day 5 retinal photoreceptor layer cacna1fa expression mislocalised, abnormal
Days 14-20 retinal outer plexiform layer cacna1fa expression decreased amount, abnormal
Days 14-20 retinal photoreceptor layer cacna1fa expression mislocalised, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Investigative ophthalmology & visual science for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Invest. Ophthalmol. Vis. Sci.