Figure 6

Neurons produced by dnmt1^−/− RSCs fail to integrate into the neural retina. A Experimental paradigm depicting BrdU incorporation from 3 to 3.5dpf. Fixations occurred at 3.5 and 5dpf. B Diagram of the four retinal domains (CMZ, GCL, INL, and ONL) whose colors correlate with the data presented in G. C–F Transverse sections of BrdU pulses from 3 to 3.5dpf (C,E) and pulse-chase assay from 3 to 5dpf (D, F) (Siblings: A, B; dnmt1^−/−C, D). Nuclei labeled with DAPI (gray). Cells in S-phase indicated by BrdU incorporation (magenta). Mitotic cells are labeled by a pH3(ser10) antibody (cyan). G Proportion of BrdU + cells located in each retinal layer at 3.5dpf and 5dpf of dnmt1^−/− and control larvae. H Proportion of total BrdU⁺ cells within the central retina of the pulse-chase experiment. White dotted lines designate the CMZ (C–F). Yellow arrows = BrdU⁺ nuclei outside the CMZ (C, E). Scale bars: 20 μm. *p < 0.05, **p < 0.005, ***p < 0.0005. Dorsal is up in all images.