ZFIN ID: ZDB-FIG-200514-45
Paatero et al., 2018 - Junction-based lamellipodia drive endothelial cell rearrangements in vivo via a VE-cadherin-F-actin based oscillatory cell-cell interaction. Nature communications   9:3545 Full text @ Nat. Commun.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: Prim-15

Fig. 6 Junction elongation and JBL formation are functionally linked. a Still images from a movie of an VE-cad-Venus expressing embryo Tg(BAC(cdh5:cdh5-ts)) during anastomosis in the DLAV (around 32 hpf), in the presence of DMSO (1%), Latrunculin B (150 ng ml−1) or NSC23766 (900 µM). b Scatter plot quantitation of the duration of the JBL. DMSO, n = 50 (6 movies); Latrunculin B, n = 13 (5 movies); NSC23766, n = 49 (10 movies); black lines show median values. Non-parametric Kruskal–Wallis statistical test was used. c Confocal images of a Tg(BAC(cdh5:cdh5-ts)) embryo during junctional elongation after DLAV anastomosis. Top panels t = 0 and bottom panels after 1 h incubation. d Quantification of the junctional elongation velocity in the presence of different chemicals using Tg(BAC(cdh5:cdh5-ts)) embryos. DMSO (1%), n = 29 junctions (11 embryos); Latrunculin B (150 ng ml−1), n = 21 (6 embryos); NSC23766 (300 µM), n = 41 (11 embryos). Dotted line indicated no movement observed, black lines are medians. Non-parametric Kruskal–Wallis statistical test was used. e Confocal images of anastomosing DLAV of EGFP-ZO1 embryos (Tg(fli:Gal4ffubs3;UAS:EGFP-hZO1ubs5)) treated with DMSO or NSC23766. Scale bar 10 µm

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ubs3Tg; ubs5Tg chemical treatment: NSC 23766 Prim-15 blood vessel endothelial cell cell-cell junction organization process quality, abnormal
Prim-15 dorsal longitudinal anastomotic vessel cell-cell junction organization process quality, abnormal
uq11bhTg chemical treatment: latrunculin B Prim-15 blood vessel endothelial cell cell-cell junction organization process quality, abnormal
Prim-15 dorsal longitudinal anastomotic vessel cell-cell junction organization process quality, abnormal
uq11bhTg chemical treatment: NSC 23766 Prim-15 blood vessel endothelial cell cell-cell junction organization process quality, abnormal
Prim-15 dorsal longitudinal anastomotic vessel cell-cell junction organization process quality, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.