ZFIN ID: ZDB-FIG-190604-63
Paatero et al., 2018 - Junction-based lamellipodia drive endothelial cell rearrangements in vivo via a VE-cadherin-F-actin based oscillatory cell-cell interaction. Nature communications   9:3545 Full text @ Nat. Commun.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: Prim-15

Fig. 5

Distinct dynamics of VE-cadherin, F-actin and ZO1 during JBL formation. ab Still images (Supplementary Movie 5) of an embryo showing the DLAV around 32 hpf in an embryo expressing both mRuby2-UCHD and VE-cad-Venus Tg(fli:Gal4ffubs3;UAS:mRuby2-UCHDubs20;BAC(cdh5:cdh5-Venus)). b A time series magnification of the inset in a. Individual channels are shown in inversed contrast. Similar observations were made in 11 movies. Open arrow head points to established junctions and black arrowhead to pioneering junction. c and d) Still images of an embryo showing DLAV around 32 hpf (Supplementary Movie 5) in an embryo expressing EGFP-ZO1 and mRuby2-UCHD (Tg(fli:Gal4ffubs3;UAS:mRuby2-UCHDubs20;UAS:EGFP-hZO1ubs5)). Imaged at rate of 12 s/stack. Similar observations were made in 9 movies. Open arrow head points to established junctions and black arrowhead to pioneering junction. e Images of endothelial cells in a VE-cad-Venus expressing embryo injected with mCherry-ZO1 encoding plasmid Tg(BAC(cdh5:cdh5-ts)); fli1ep:mCherry-ZO1)) (n = 7 embryos). f Close-up from panel e. Both channels are shown in inverted contrast. Scale bars 1 µm (bd) and 10 µm (ae)

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP ubs20Tg; ubs3Tg; ubs5Tg standard conditions Prim-15 blood vessel endothelial cell cell-cell junction IFL
mRuby ubs20Tg; ubs3Tg; ubs5Tg standard conditions Prim-15 blood vessel endothelial cell cell-cell junction IFL
ubs20Tg; ubs3Tg; uq11bhTg standard conditions Prim-15 blood vessel endothelial cell cell-cell junction IFL
Venus ubs20Tg; ubs3Tg; uq11bhTg standard conditions Prim-15 blood vessel endothelial cell cell-cell junction IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.