ZFIN ID: ZDB-FIG-200514-42
Paatero et al., 2018 - Junction-based lamellipodia drive endothelial cell rearrangements in vivo via a VE-cadherin-F-actin based oscillatory cell-cell interaction. Nature communications   9:3545 Full text @ Nat. Commun.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Prim-15

Fig. 2 Active VE-cad and F-actin behavior of junction-based lamellipodia. a, b Still images from a movie (Supplementary Movie 2) of a VE-cad-Venus expressing embryo Tg(BAC(cdh5:cdh5-ts)), showing the DLAV at 30 hpf in inversed contrast. b A magnification of the inset in a. Arrows point to JBL. c, d Still images from a movie (Supplementary Movie 3) of a EGFP-UCHD expressing embryo (Tg(fli:Gal4ffubs3, UAS:EGFP-UCHDubs18)) showing the DLAV at 30 hpf in inversed contrast. d A magnification of the inset in c. Arrows point to JBL. e Scatter plot of quantitation of the duration of the JBL with the VE-cad-Venus transgene (n = 48 in 6 embryos) and EGFP-UCHD movies (n = 74 in 6 embryos), red line represents the median. f Quantitation of JBL angle in the DLAV in respect to the blood vessel axis (0°) using the EGFP-UCHD transgene (n = 103 from 6 embryos) or Cdh5-Venus transgene (n = 41 from 5 embryos). Scale bars 5 µm

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP ubs18Tg; ubs3Tg standard conditions Prim-15 blood vessel endothelial cell lamellipodium IFL
Venus uq11bhTg standard conditions Prim-15 blood vessel endothelial cell cell-cell junction IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.