ZFIN ID: ZDB-FIG-200413-53
Chestnut et al., 2019 - Zebrafish etv2 knock-in line labels vascular endothelial and blood progenitor cells. Developmental dynamics : an official publication of the American Association of Anatomists   249(2):245-261 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 14-19 somites to 20-25 somites

Fig. 3 A comparison of etv2 ci32Gt+/−; UAS:GFP, Tg(−2.3etv2:GFP), TgBAC(etv2:GFP) fluorescence pattern and etv2 mRNA expression analyzed by in situ hybridization (ISH) at the 14‐21 somite stages. A‐C, GFP fluorescence or, D, etv2 mRNA expression is apparent in bilaterally located vascular endothelial progenitors which have started migrating toward the midline at the 14‐15‐somite stages (arrowheads). Strong nonspecific GFP expression in the neural tube (NT) is apparent in TgBAC(etv2:GFP) embryos. E‐H, GFP‐positive vascular endothelial progenitors at the 18‐19‐somite stages are coalescing at the midline into vascular cords (arrowheads, E,F). Similar etv2 expression pattern is observed by ISH analysis, H. I‐L, GFP expression at the 20‐21‐somite stages is apparent in the forming axial vasculature (arrowheads), and nonspecific expression is apparent in the neural tube, K. Similar etv2 expression pattern is apparent from ISH analysis, L. A‐H, dorsal or dorsolateral view; I‐L, lateral view, anterior is to the left

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP ci1Tg standard conditions 14-19 somites embryonic blood vessel endothelial progenitor cell IFL
14-19 somites notochord IFL
20-25 somites notochord IFL
20-25 somites vascular cord IFL
etsrpci32Gt/+; nkuasgfp1aTg standard conditions 14-19 somites embryonic blood vessel endothelial progenitor cell IFL
20-25 somites vascular cord IFL
zf372Tg standard conditions 14-19 somites embryonic blood vessel endothelial progenitor cell IFL
20-25 somites vascular cord IFL
etsrp WT standard conditions 14-19 somites embryonic blood vessel endothelial progenitor cell ISH
20-25 somites vascular cord ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.