ZFIN ID: ZDB-FIG-200413-52
Chestnut et al., 2019 - Zebrafish etv2 knock-in line labels vascular endothelial and blood progenitor cells. Developmental dynamics : an official publication of the American Association of Anatomists   249(2):245-261 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Prim-5

Fig. 4 A comparison of etv2 ci32Gt+/−; UAS:GFP, TgBAC(etv2:GFP), Tg(−2.3etv2:GFP) fluorescence pattern (in kdrl:mCherry background, top row) and etv2 mRNA expression analyzed by in situ hybridization (ISH) at 24 hpf. etv2 ci32Gt+/−; UAS:GFP expression is apparent throughout the entire vasculature, red blood cells (RBC), and macrophages (MF). TgBAC(etv2:GFP) shows similar expression pattern, and also shows nonspecific expression in the neural tube. A‐C, Merged mCherry and GFP channels; D‐F, GFP channel; G,H, magnified images of the trunk region in, A and B. I, ISH analysis for etv2 mRNA expression at 24 hpf. DA, dorsal aorta; PCV, posterior cardinal vein; ISV, intersegmental vessels

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP ci1Tg; ci5Tg standard conditions Prim-5 cranial vasculature IFL
Prim-5 dorsal aorta IFL
Prim-5 intersegmental vessel IFL
Prim-5 macrophage IFL
Prim-5 notochord IFL
Prim-5 nucleate erythrocyte IFL
Prim-5 posterior cardinal vein IFL
ci5Tg; zf372Tg standard conditions Prim-5 cranial vasculature IFL
Prim-5 dorsal aorta IFL
Prim-5 intersegmental vessel IFL
Prim-5 macrophage IFL
Prim-5 nucleate erythrocyte IFL
Prim-5 posterior cardinal vein IFL
etsrpci32Gt/+; ci5Tg/+; nkuasgfp1aTg standard conditions Prim-5 blood vessel endothelial cell IFL
Prim-5 cranial vasculature IFL
Prim-5 dorsal aorta IFL
Prim-5 intersegmental vessel IFL
Prim-5 macrophage IFL
Prim-5 nucleate erythrocyte IFL
Prim-5 posterior cardinal vein IFL
etsrp WT standard conditions Prim-5 dorsal aorta ISH
Prim-5 intersegmental vessel ISH
Prim-5 posterior cardinal vein ISH
mCherry ci1Tg; ci5Tg standard conditions Prim-5 dorsal aorta IFL
Prim-5 intersegmental vessel IFL
ci5Tg; zf372Tg standard conditions Prim-5 dorsal aorta IFL
Prim-5 intersegmental vessel IFL
etsrpci32Gt/+; ci5Tg/+; nkuasgfp1aTg standard conditions Prim-5 dorsal aorta IFL
Prim-5 intersegmental vessel IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.