ZFIN ID: ZDB-FIG-200314-77
Hau et al., 2020 - Maternal Larp6 controls oocyte development, chorion formation and elevation. Development (Cambridge, England)   147(4) Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Anatomical Term:
Stage: Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage Range: 1k-cell to Day 5

Fig. 3.

Maternal effect of larp6a−/− and larp6a−/−;larp6b−/− on oogenesis. (A) Bright-field images of 5 dpf larvae from the indicated crosses. Fish are shown with anterior towards the left and dorsal upwards. Inflated swim bladders show that larvae have swimming and swallowing capacity. (B) Slow myosin immunodetection in 48 hpf larvae from a larp6a−/−;larp6b−/− female crossed to a larp6a+/−;larp6b+/− male reveal no differences in slow muscle fibre formation. Images are centred on somite 17/18 and the graph shows the number of slow fibres per myotome±s.d., tested using Welch's ANOVA on SPSS. (C) Bright-field images of lays at 3 hpf from wild-type, larp6a−/−, larp6b−/− and larp6a−/−;larp6b−/− females (upper panels) or males (lower panels) crossed to wild-type AB. Embryos from males of any genotype or wild-type females show a fully elevated chorion (ch), translucent and smooth yolk (y), tall and symmetrical blastodisc (bd), large chorion diameter (brackets), and large subchorionic space (asterisk). All embryos from maternal larp6a−/− mutants have reduced chorion diameter, reduced subchorionic space (black lines) surrounding a yolk cell of unaltered size (red lines) but more opaque and uneven. Lays from larp6b−/− females are indistinguishable from wild type. Lays from larp6a−/−;larp6b−/− females are like larp6a−/− embryos, but have even smaller chorion diameter (brackets). (D) Chorion diameter cumulative frequency distribution curves. Light colours indicate individual clutches strong colour indicates the mean for each genotype. (E) Average of the mean chorion diameters of n clutches from separate females of indicated maternal genotype±s.d. One-way ANOVA with Tukey's post-hoc test on SPSS. Scale bars: 1 mm in A,C; 100 µm in B.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-F59 IHC larp6akg139/+; larp6bkg153/+(AB) standard conditions Long-pec myotome slow muscle cell
IHC larp6akg139/+; larp6bkg153/kg153(AB) standard conditions Long-pec myotome slow muscle cell
IHC larp6akg139/kg139; larp6bkg153/+(AB) standard conditions Long-pec myotome slow muscle cell
IHC larp6akg139/kg139; larp6bkg153/kg153(AB) standard conditions Long-pec myotome slow muscle cell
Phenotype Details
Fish Conditions Stage Phenotype
larp6akg139/+(AB) standard conditions 1k-cell chorion decreased diameter, abnormal
1k-cell chorion increased fragility, abnormal
larp6akg139/+(AB) standard conditions 1k-cell chorion normal diameter, normal
1k-cell chorion normal fragility, normal
larp6akg139/+(AB) standard conditions 1k-cell chorion development decreased process quality, abnormal
larp6akg139/+(AB) standard conditions 1k-cell chorion development normal process quality, normal
larp6akg139/+(AB) standard conditions 1k-cell oogenesis decreased process quality, abnormal
larp6akg139/+(AB) standard conditions 1k-cell oogenesis normal process quality, normal
larp6akg139/+(AB) standard conditions 1k-cell yolk normal size, normal
larp6akg139/+(AB) standard conditions 1k-cell yolk normal size, normal
larp6akg139/+(AB) standard conditions 1k-cell yolk opaque, abnormal
larp6akg139/+(AB) standard conditions 1k-cell yolk transparent, normal
larp6akg139/+(AB) standard conditions Day 5 swim bladder inflated, normal
larp6akg139/+; larp6bkg153/+(AB) standard conditions 1k-cell chorion decreased diameter, exacerbated
1k-cell chorion increased fragility, abnormal
larp6akg139/+; larp6bkg153/+(AB) standard conditions 1k-cell chorion normal diameter, normal
1k-cell chorion normal fragility, normal
larp6akg139/+; larp6bkg153/+(AB) standard conditions 1k-cell chorion development decreased process quality, exacerbated
larp6akg139/+; larp6bkg153/+(AB) standard conditions 1k-cell chorion development normal process quality, normal
larp6akg139/+; larp6bkg153/+(AB) standard conditions 1k-cell oogenesis decreased process quality, exacerbated
larp6akg139/+; larp6bkg153/+(AB) standard conditions 1k-cell oogenesis normal process quality, normal
1k-cell yolk normal size, normal
larp6akg139/+; larp6bkg153/+(AB) standard conditions 1k-cell yolk normal size, normal
1k-cell yolk opaque, abnormal
larp6akg139/+; larp6bkg153/+(AB) standard conditions 1k-cell yolk transparent, normal
larp6akg139/+; larp6bkg153/+(AB) standard conditions Long-pec myotome has normal numbers of parts of type slow muscle cell, normal
Long-pec myotome slow muscle cell morphology, normal
Long-pec slow muscle cell skeletal muscle tissue development normal process quality, normal
larp6akg139/+; larp6bkg153/kg153(AB) standard conditions Long-pec myotome has normal numbers of parts of type slow muscle cell, normal
Long-pec myotome slow muscle cell morphology, normal
Long-pec slow muscle cell skeletal muscle tissue development normal process quality, normal
larp6akg139/+; larp6bkg153/kg153(AB) standard conditions Day 5 swim bladder inflated, normal
larp6akg139/kg139(AB) standard conditions Day 5 swim bladder inflated, normal
larp6akg139/kg139; larp6bkg153/+(AB) standard conditions Long-pec myotome has normal numbers of parts of type slow muscle cell, normal
Long-pec myotome slow muscle cell morphology, normal
Long-pec slow muscle cell skeletal muscle tissue development normal process quality, normal
larp6akg139/kg139; larp6bkg153/kg153(AB) standard conditions Long-pec myotome has normal numbers of parts of type slow muscle cell, normal
Long-pec myotome slow muscle cell morphology, normal
Long-pec slow muscle cell skeletal muscle tissue development normal process quality, normal
larp6akg139/kg139; larp6bkg153/kg153(AB) standard conditions Day 5 swim bladder inflated, normal
larp6bkg153/+(AB) standard conditions 1k-cell chorion normal diameter, normal
larp6bkg153/+(AB) standard conditions 1k-cell chorion normal diameter, normal
larp6bkg153/+(AB) standard conditions 1k-cell chorion normal fragility, normal
larp6bkg153/+(AB) standard conditions 1k-cell chorion normal fragility, normal
larp6bkg153/+(AB) standard conditions 1k-cell chorion development normal process quality, normal
larp6bkg153/+(AB) standard conditions 1k-cell chorion development normal process quality, normal
larp6bkg153/+(AB) standard conditions 1k-cell oogenesis normal process quality, normal
larp6bkg153/+(AB) standard conditions 1k-cell oogenesis normal process quality, normal
larp6bkg153/+(AB) standard conditions 1k-cell yolk normal size, normal
larp6bkg153/+(AB) standard conditions 1k-cell yolk normal size, normal
larp6bkg153/+(AB) standard conditions 1k-cell yolk transparent, normal
larp6bkg153/+(AB) standard conditions 1k-cell yolk transparent, normal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development