ZFIN ID: ZDB-FIG-200314-76
Hau et al., 2020 - Maternal Larp6 controls oocyte development, chorion formation and elevation. Development (Cambridge, England)   147(4) Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Anatomical Term:
Stage: Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-5 to Adult

Fig. 2.

Zygotic Larp6 mutants appear wild type. (A) Bright-field images of 5 dpf larvae from wild-type and incrosses of single and double heterozygous carriers. Fish are shown anterior towards the left and dorsal upwards with genotyped mutants above their respective wild-type siblings (wt sib). Inflated swim bladders show that larvae have swimming and swallowing capacity. (B) Adults derived from incrosses of larp6a+/ (n=52), larp6b+/ (n=62) and larp6a+/−;larp6b+/− (n=61) fish were co-reared, then genotyped at 5 mpf showing the expected Mendelian ratios. Fish numbers are above each bar. (C) Slow myosin immunofluorescence of 24 hpf embryos. Images of wild type and mutant centred on somite 17/18 reveal no differences in slow muscle fibre formation. (D) Length and weight of sibling individuals from larp6a+/−, larp6b+/− and larp6a+/−;larp6b+/− in-crosses determined at 5 mpf show no significant difference between genotypes. Large symbols reflect means for each sex and genotype±s.e.m. Individuals data points are also plotted. Scale bars: 1 mm in A; 100 µm in C.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-F59 IHC AB standard conditions Prim-5 myotome slow muscle cell
IHC larp6akg139/kg139(AB) standard conditions Prim-5 myotome slow muscle cell
Phenotype Details
Fish Conditions Stage Phenotype
larp6akg139/kg139(AB) standard conditions Prim-5 myotome slow muscle cell morphology, normal
Prim-5 slow muscle cell skeletal muscle tissue development normal process quality, normal
Day 5 swim bladder inflated, normal
Adult whole organism normal length, normal
Adult whole organism weight, normal
larp6akg139/kg139; larp6bkg153/kg153(AB) standard conditions Day 5 swim bladder inflated, normal
Adult whole organism normal length, normal
Adult whole organism weight, normal
larp6bkg153/kg153(AB) standard conditions Day 5 swim bladder inflated, normal
Adult whole organism normal length, normal
Adult whole organism weight, normal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development