Figure 7

Electrophysiological antiseizure analysis of TMC-120A and TMC-120B in the zebrafish PTZ seizure model. Noninvasive local field potential recordings from the optic tectum of larvae pre-exposed to vehicle (VHC) and pentylenetetrazole (PTZ), VHC only, compound and PTZ, or compound and VHC. Larvae were incubated with 20 µg/mL TMC-120A or TMC-120B for 2 h, conform with the maximum tolerated concentrations and incubation time used in the behavioral assay. Larvae are considered to possess epileptiform brain activity when three or more epileptiform events occurred during a 10-min recording (A). Epileptiform discharges are quantified by the number (mean ±SD) (B) and cumulative duration (mean ± SD) (C) of events per 10-min recording. Number of replicate wells per condition: 19 larvae were used for VHC + PTZ controls, 16 larvae were used for VHC + VHC controls, 13–14 larvae were used for compound + PTZ conditions, and 12 larvae were used for compound + VHC conditions. Statistical analysis: (A) Fisher’s exact test with Bonferroni posttest, (B,C) Kruskal–Wallis test with Dunn’s multiple comparison test (data did not pass the Shapiro–Wilk normality test) (GraphPad Prism 5, San Diego, CA, USA). Significance levels: * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001.