Figure 6

Behavioral antiseizure analysis of TMC-120A, TMC-120B, and structural analogues in the zebrafish PTZ seizure model. Antiseizure activity of TMC-120A (A,B), TMC-120B (C,D), and their structural analogues (TMC-120C, penicisochroman G, and ustusorane B) (E,F) in the zebrafish pentylenetetrazole (PTZ) seizure model after 2 h of incubation, respectively. PTZ-induced seizure-like behavior is expressed as mean actinteg units per 5 min (±SEM) during the 30-min recording period (A,C,E) and over consecutive time intervals (B,D,F). (A–D) Means are pooled from three independent experiments with each 10–12 replicate wells per vehicle (VHC) + PTZ and compound + PTZ condition, and 6–12 replicate wells per VHC + VHC and compound + VHC condition. (E–F) Data are pooled from two single experiments. Number of replicate wells per condition: 21–22 replicate wells for VHC + PTZ and VHC + VHC conditions, and 8–11 replicate wells for compound + PTZ conditions. Statistical analysis: (A,C,E) one-way ANOVA with Dunnett’s multiple comparison test, (B,D,F) two-way ANOVA with Bonferroni posttests (GraphPad Prism 5, San Diego, CA, USA). Significance levels: * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001.