ZFIN ID: ZDB-FIG-191230-1704
Soman et al., 2019 - Restriction of mitochondrial calcium overload by mcu inactivation renders neuroprotective effect in Zebrafish models of Parkinson's disease. Biology Open   8(10) Full text @ Biol. Open
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Anatomical Term:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Protruding-mouth

Fig. 6.

mcu inactivation renders neuroprotection against MPTP. (A) Representative images of MPTP-untreated wt (a), pink1−/− (c), (pink1; mcu)−/− (e), mcu−/− (g) and MPTP-treated wt (b), pink1−/− (d), (pink1; mcu)−/− (f) and mcu−/− (h) 3 dpf larvae after WISH using TH-specific riboprobe. MPTP-treated wt (b) and pink1−/− (d) zebrafish larvae were most susceptible to MPTP toxicity while MPTP-untreated (pink1; mcu)−/− (e) and mcu−/− (g) were most resistant to MPTP toxicity. (B) Graphical representation of chromogenic WISH. There was a significant decrease (***P<0.001) in number of dopaminergic neurons in pink1−/− zebrafish treated with MPTP when compared to untreated pink1−/− zebrafish. MPTP-treated (pink1; mcu)−/− zebrafish showed a significant increase (***P<0.001) in number of dopaminergic neurons when compared to MPTP-treated pink1−/− zebrafish. Statistical analysis with one-way anova and post hoc analysis using Tukey's multiple comparison test of two different experiments with n=20. (C) Kaplan–Meier survival curves depicting survival rate for 5 days of wt, pink1−/−, mcu−/− and (pink1; mcu)−/− zebrafish treated with 25 µg ml-1 MPTP (n=100). Scale bars:100 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
th AB standard conditions Protruding-mouth diencephalon dopaminergic neuron ISH
AB chemical treatment by environment: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine Protruding-mouth diencephalon dopaminergic neuron ISH
mcush214/sh214 standard conditions Protruding-mouth diencephalon dopaminergic neuron ISH
mcush214/sh214 chemical treatment by environment: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine Protruding-mouth diencephalon dopaminergic neuron ISH
mcush214/sh214 ; pink1sh397/sh397 standard conditions Protruding-mouth diencephalon dopaminergic neuron ISH
mcush214/sh214 ; pink1sh397/sh397 chemical treatment by environment: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine Protruding-mouth diencephalon dopaminergic neuron ISH
pink1sh397/sh397 standard conditions Protruding-mouth diencephalon dopaminergic neuron ISH
pink1sh397/sh397 chemical treatment by environment: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine Protruding-mouth diencephalon dopaminergic neuron ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB chemical treatment by environment: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine Protruding-mouth diencephalon has fewer parts of type dopaminergic neuron, abnormal
mcush214/sh214 standard conditions Protruding-mouth diencephalon has normal numbers of parts of type dopaminergic neuron, normal
mcush214/sh214 chemical treatment by environment: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine Protruding-mouth diencephalon has number of dopaminergic neuron, ameliorated
mcush214/sh214 ; pink1sh397/sh397 standard conditions Protruding-mouth diencephalon has number of dopaminergic neuron, ameliorated
mcush214/sh214 ; pink1sh397/sh397 chemical treatment by environment: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine Protruding-mouth diencephalon has number of dopaminergic neuron, ameliorated
pink1sh397/sh397 standard conditions Protruding-mouth diencephalon has fewer parts of type dopaminergic neuron, abnormal
pink1sh397/sh397 chemical treatment by environment: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine Protruding-mouth diencephalon has fewer parts of type dopaminergic neuron, exacerbated
Acknowledgments:
ZFIN wishes to thank the journal Biology Open for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Biol. Open