ZFIN ID: ZDB-FIG-191230-1701
Soman et al., 2019 - Restriction of mitochondrial calcium overload by mcu inactivation renders neuroprotective effect in Zebrafish models of Parkinson's disease. Biology Open   8(10) Full text @ Biol. Open
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Term:
Stage Range: 1-cell to Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage Range: 1-cell to Protruding-mouth

Fig. 3.

Dopaminergic neurons are rescuedafter deleting mcu in pink1−/− zebrafish. (A–D) Representative images of wt (A), pink1−/− (B), mcu−/− (C) and (pink1; mcu)−/− (D) 3 dpf larvae after WISH using TH-specific riboprobe. (E–F) Representative images of wt (E), pink1−/− (F), mcu−/− (G) and (pink1; mcu)−/− (H) 3 dpf larvae after whole-mount FISH using TH-specific riboprobe and TSA/Cy3-based signal amplification. There was a significant decrease (P<0.001) in number of dopaminergic neurons in pink1−/− zebrafish when compared to wt. In (pink1; mcu)−/− zebrafish there was a significant increase (P<0.05) in number of dopaminergic neurons when compared to pink1−/− zebrafish. (I–L) Representative images of wt (I), pink1−/− (J), mcu−/− (K) and (pink1; mcu)−/− (L) 3 dpf larvae after immunohistochemistry using TH-specific antibody. Arrowheads show absence of dopaminergic neurons. (M) Graphical representation of chromogenic WISH. There was a significant decrease (**P<0.01) in number of dopaminergic neurons in pink1−/− zebrafish when compared to wt. In (pink1; mcu)−/− zebrafish, there was a significant increase (***P<0.001) in number of dopaminergic neurons when compared to pink1−/− zebrafish. (N) Graphical representation of immunohistochemistry. There was a significant decrease (***P<0.001) in number of dopaminergic neurons in pink1−/− zebrafish when compared to wt. In (pink1; mcu)−/− zebrafish there was a significant increase (***P<0.001) in number dopaminergic neurons when compared to pink1−/− zebrafish. The mean number of diencephalic dopaminergic neurons for wt, pink1−/−, mcu−/− and (pink1; mcu)−/− was calculated over three independent experiments (n=10 embryos per genotype and experiment). Scale bars: 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
th Ab3-th AB standard conditions 1-cell to Protruding-mouth diencephalon dopaminergic neuron IHC
1-cell to Protruding-mouth diencephalon dopaminergic neuron ISH
Ab3-th mcush214/sh214(AB) standard conditions 1-cell to Protruding-mouth diencephalon dopaminergic neuron IHC
1-cell to Protruding-mouth diencephalon dopaminergic neuron ISH
Ab3-th mcush214/sh214 ; pink1sh397/sh397(AB) standard conditions 1-cell to Protruding-mouth diencephalon dopaminergic neuron IHC
1-cell to Protruding-mouth diencephalon dopaminergic neuron ISH
pink1sh397/sh397(AB) standard conditions 1-cell to Protruding-mouth diencephalon dopaminergic neuron ISH
Ab3-th 1-cell to Protruding-mouth diencephalon dopaminergic neuron IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
mcush214/sh214(AB) standard conditions 1-cell - Protruding-mouth diencephalon has normal numbers of parts of type dopaminergic neuron, normal
mcush214/sh214 ; pink1sh397/sh397(AB) standard conditions 1-cell - Protruding-mouth diencephalon has number of dopaminergic neuron, ameliorated
pink1sh397/sh397(AB) standard conditions 1-cell - Protruding-mouth diencephalon has fewer parts of type dopaminergic neuron, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Biology Open for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Biol. Open