ZFIN ID: ZDB-FIG-191230-1462
Ye et al., 2019 - Marcksb plays a key role in the secretory pathway of zebrafish Bmp2b. PLoS Genetics   15:e1008306 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Shield
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Shield

Fig 2 Marcksb is required for activation of BMP signaling.

(A) WISH analysis showed that the transcriptional levels of downstream targets of BMP signaling—szl and ved were decreased in marcksb morphants. (B) The percentage of embryos with normal or decreased expression. “n” represents the number of embryos we observed. (C) The overall intensity of p-Smad1/5/9 at ventral region in marcksb morphants was dramatically decreased when compared with wildtype. (D) The normalized fluorescent intensity of P-Smad1/5/9. Error bars of light blue and light red show S.E.M. (E) Knockdown of marcksb could partially rescue the ventralization phenotype caused by injection of bmp2b mRNA (5 pg bmp2b mRNA per embryo). The statistical data are shown in the bar graphs with the number of observed embryos. “n” represents the number of embryos we observed. (F) Overexpression of bmp2b caused dramatic dorsal expansion of szl and ved expression while knockdown of marcksb in the bmp2b-overexpressed embryos partially restored their expression patterns. (G) The percentage of embryos with robust-increased or dorsal-inhibited expression. “n” represents the number of embryos we observed.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
szl AB standard conditions Shield whole organism ventral region ISH
AB + MO1-marcksb standard conditions Shield whole organism ventral region ISH
ved AB standard conditions Shield whole organism ventral region ISH
AB + MO1-marcksb standard conditions Shield whole organism ventral region ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab13-smad IHC AB standard conditions Shield whole organism ventral region
IHC AB + MO1-marcksb standard conditions Shield whole organism ventral region
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-marcksb standard conditions Shield BMP signaling pathway decreased occurrence, abnormal
Shield whole organism ventral region Ab13-smad labeling decreased amount, abnormal
Shield whole organism ventral region ved expression decreased distribution, abnormal
Shield whole organism ventral region szl expression decreased distribution, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.