Marcksb is required for activation of BMP signaling.
(A) WISH analysis showed that the transcriptional levels of downstream targets of BMP signaling—szl and ved were decreased in marcksb morphants. (B) The percentage of embryos with normal or decreased expression. “n” represents the number of embryos we observed. (C) The overall intensity of p-Smad1/5/9 at ventral region in marcksb morphants was dramatically decreased when compared with wildtype. (D) The normalized fluorescent intensity of P-Smad1/5/9. Error bars of light blue and light red show S.E.M. (E) Knockdown of marcksb could partially rescue the ventralization phenotype caused by injection of bmp2b mRNA (5 pg bmp2b mRNA per embryo). The statistical data are shown in the bar graphs with the number of observed embryos. “n” represents the number of embryos we observed. (F) Overexpression of bmp2b caused dramatic dorsal expansion of szl and ved expression while knockdown of marcksb in the bmp2b-overexpressed embryos partially restored their expression patterns. (G) The percentage of embryos with robust-increased or dorsal-inhibited expression. “n” represents the number of embryos we observed.