Marcksb plays a key role in the secretory pathway of zebrafish Bmp2b
- Ye, D., Wang, X., Wei, C., He, M., Wang, H., Wang, Y., Zhu, Z., Sun, Y.
- PLoS Genetics 15: e1008306 (Journal)
- Registered Authors
- He, Mudan, Sun, Yonghua, Wei, Changyong, Ye, Ding, Zhu, Zuoyan
- MeSH Terms
- Body Patterning/physiology
- Bone Morphogenetic Protein 2/metabolism*
- Embryo, Nonmammalian/metabolism
- Embryonic Development/genetics
- Embryonic Development/physiology
- HSP70 Heat-Shock Proteins/metabolism
- Intracellular Signaling Peptides and Proteins/metabolism*
- Secretory Pathway
- Signal Transduction
- Transforming Growth Factor beta/metabolism
- Zebrafish Proteins/metabolism*
- 31545789 Full text @ PLoS Genet.
Ye, D., Wang, X., Wei, C., He, M., Wang, H., Wang, Y., Zhu, Z., Sun, Y. (2019) Marcksb plays a key role in the secretory pathway of zebrafish Bmp2b. PLoS Genetics. 15:e1008306.
During vertebrate early embryogenesis, the ventral development is directed by the ventral-to-dorsal activity gradient of the bone morphogenetic protein (BMP) signaling. As secreted ligands, the extracellular traffic of BMP has been extensively studied. However, it remains poorly understood that how BMP ligands are secreted from BMP-producing cells. In this work, we show the dominant role of Marcksb controlling the secretory process of Bmp2b via interaction with Hsp70 in vivo. We firstly carefully characterized the role of Marcksb in promoting BMP signaling during dorsoventral axis formation through knockdown approach. We then showed that Marcksb cell autonomously regulates the trafficking of Bmp2b from producing cell to the extracellular space and both the total and the extracellular Bmp2b was decreased in Marcksb-deficient embryos. However, neither the zygotic mutant of marcksb (Zmarcksb) nor the maternal zygotic mutant of marcksb (MZmarcksb) showed any defects of dorsalization. In contrast, the MZmarcksb embryos even showed increased BMP signaling activity as measured by expression of BMP targets, phosphorylated Smad1/5/9 levels and imaging of Bmp2b, suggesting that a phenomenon of "genetic over-compensation" arose. Finally, we revealed that the over-compensation effects of BMP signaling in MZmarcksb was achieved through a sequential up-regulation of MARCKS-family members Marcksa, Marcksl1a and Marcksl1b, and MARCKS-interacting protein Hsp70.3. We concluded that the Marcksb modulates BMP signaling through regulating the secretory pathway of Bmp2b.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes