ZFIN ID: ZDB-FIG-191230-1361
Palasin et al., 2019 - Abnormal development of zebrafish after knockout and knockdown of ribosomal protein L10a. Scientific Reports   9:18130 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Figure 8

(a) Whole-mount in situ hybridization showing the expression of PGC marker genes, including nanos1 and vasa, after morpholino injection at 25 hpf. The reduction of nanos1 and vasa expression intensity was obviously observed in MOaug-injected embryos. The nanos1 expression was also decreased in MOsp-injected embryos and it was recovered when mRNA was co-injected. The expression levels of nanos1 (b) and vasa (c) were graded as the normal and abnormal percentage. The mean pixel intensity of nanos1 and vasa gene expression of the normal embryos were 24.7 ± 8.7 and 22.6 ± 8.2, respectively. An abnormal level of the gene expression was taken as one with the value less than the mean minus one standard deviation of the gene expression of the wild-type, i.e., less than 16 for nanos1 and 14.4 for vasa gene. The number of animals quantified in each group are shown on top of the bars.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ddx4 AB standard conditions Prim-5 primordial germ cell ISH
AB + MO1-rpl10a standard conditions Prim-5 primordial germ cell ISH
AB + MO1-rps19 standard conditions Prim-5 primordial germ cell ISH
AB + MO2-rpl10a standard conditions Prim-5 primordial germ cell ISH
nanos1 AB standard conditions Prim-5 primordial germ cell ISH
AB + MO1-rpl10a standard conditions Prim-5 primordial germ cell ISH
AB + MO1-rps19 standard conditions Prim-5 primordial germ cell ISH
AB + MO2-rpl10a standard conditions Prim-5 primordial germ cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-rpl10a standard conditions Prim-5 primordial germ cell nanos1 expression decreased amount, abnormal
Prim-5 primordial germ cell ddx4 expression decreased amount, abnormal
AB + MO2-rpl10a standard conditions Prim-5 primordial germ cell nanos1 expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.