ZFIN ID: ZDB-FIG-190822-25
Fischer et al., 2019 - FoxH1 represses miR-430 during early embryonic development of zebrafish via non-canonical regulation. BMC Biology   17:61 Full text @ BMC Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 30%-epiboly to Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: 30%-epiboly to Prim-5

Fig. 4

FHD-GFP interferes with severity of the MZsur mutant phenotype. adWild-type (ab) and MZsur mutant (cd) embryos at 24hpf. col2a1a in-situ staining in wild-type (un-injected control (a) or injected with FHD-GFP (b)) shows wild-type notochord of expected width (white brackets in enlarged sections a′ and b′). In uninjected MZsur embryos the width is reduced (c′). Injection of FHD-GFP in MZsur mutants enhances the phenotype (d/d′; note reduced size and additionally discontinuity of staining). el foxa2 in-situ hybridizations show reduction of axial mesoderm formation in MZsurmutants. Injection of FHD-GFP causes a broadened axial signal in 50% of wild type embryos, but no reduction of axial cells (f), and strengthens the effect in 60% of the MZsur mutants (h). dre-miR-430 morpholinos (MOs) massively reduce axial foxa2 signals in both genotypes (lk). Co-injection of FHD-GFP and MOs also results in a decreased staining (jl) when compared to FHD-GFP injection (fh). Percentage of embryos showing the same phenotype as in the image is given (upper right). mt sox17 in-situ hybridizations show only slight reduction of endoderm after injection of MOs (qt). Number of forerunner cells (black arrow) is reduced in MZsurcontrol and after MOs injections (oqs). FHD-GFP lead to complete loss of forerunner cells (black arrow) in the majority of MZsur embryos (pt; see also Additional file : Statistical analysis of forerunner cells). Numbers of sox17-positive cells seen in dorsal view and standard deviation are given (upper right) as well as number of analyzed embryos (n) (lower right). Size bars: 200 μm. γ value was changed to 0.8 in each picture
 

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
col2a1a WT standard conditions Prim-5 notochord ISH
foxh1m768/m768 standard conditions Prim-5 notochord ISH
foxa2 WT standard conditions 30%-epiboly to 90%-epiboly axial mesoderm ISH
WT + MO1-mir430a + MO1-mir430c + MO4-mir430 standard conditions 30%-epiboly to 90%-epiboly axial mesoderm ISH
foxh1m768/m768 standard conditions 30%-epiboly to 90%-epiboly axial mesoderm ISH
foxh1m768/m768 + MO1-mir430a + MO1-mir430c + MO4-mir430 standard conditions 30%-epiboly to 90%-epiboly axial mesoderm ISH
sox17 WT standard conditions 30%-epiboly to 90%-epiboly endoderm ISH
30%-epiboly to 90%-epiboly forerunner cell group ISH
WT + MO1-mir430a + MO1-mir430c + MO4-mir430 standard conditions 30%-epiboly to 90%-epiboly forerunner cell group ISH
foxh1m768/m768 standard conditions 30%-epiboly to 90%-epiboly endoderm ISH
30%-epiboly to 90%-epiboly forerunner cell group ISH
foxh1m768/m768 + MO1-mir430a + MO1-mir430c + MO4-mir430 standard conditions 30%-epiboly to 90%-epiboly forerunner cell group ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-mir430a + MO1-mir430c + MO4-mir430 standard conditions 30%-epiboly - 90%-epiboly axial mesoderm foxa2 expression decreased amount, abnormal
30%-epiboly - 90%-epiboly forerunner cell group sox17 expression decreased amount, abnormal
30%-epiboly - 90%-epiboly forerunner cell group decreased size, abnormal
foxh1m768/m768 standard conditions 30%-epiboly - 90%-epiboly forerunner cell group decreased size, abnormal
Prim-5 notochord col2a1a expression decreased amount, abnormal
Prim-5 notochord decreased width, abnormal
foxh1m768/m768 + MO1-mir430a + MO1-mir430c + MO4-mir430 standard conditions 30%-epiboly - 90%-epiboly axial mesoderm foxa2 expression decreased amount, abnormal
30%-epiboly - 90%-epiboly forerunner cell group sox17 expression decreased amount, abnormal
30%-epiboly - 90%-epiboly forerunner cell group decreased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal BMC Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Biol.