FIGURE

Fig. 3

ID

ZDB-FIG-190815-25

Publication

Kang et al., 2019 - Ankrd45 Is a Novel Ankyrin Repeat Protein Required for Cell Proliferation

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Fig. 3

Subcellular localization of ANKRD45 during mitosis. (A–D”) Subcellular localization of ANKRD45 during mitosis in human hepatoma Hep3B cells. The distribution of ANKRD45 was visualized with anti-ANKRD45 antibody (green). The cytoskeleton was stained by anti-α-tubulin antibody (red) and nuclei were labeled with DAPI (blue). (E) Hep3B cells were treated with control or four siRNAs against ANKRD45 and analyzed with Western blotting using ANKRD45 antibody or a cleaved form of the PARP antibody. The α-tubulin antibody was used as a loading control. Bottom bar graph shows the statistical results of ANKRD45 expression in control or siRNA-treated cells from the immunoblots results. (F,G) Confocal images showing the localization of ANKRD45 in the midbody during cytokinesis in control (F) or siRNA#2 (G) treated HeLa cells. (H) Quantitative analysis of relative ANKRD45 expression in the midbody ring of control or siRNA#2-treated HeLa cells. NC, negative control. Scale bars: 5 μm. *** p < 0.001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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