FIGURE

Fig. 1

ID

ZDB-FIG-190815-23

Publication

Kang et al., 2019 - Ankrd45 Is a Novel Ankyrin Repeat Protein Required for Cell Proliferation

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Fig. 1

Expression pattern of ankrd45 and cilia development in ankrd45 mutants. (A–C) Whole-mount in situ hybridization results showing the expression of ankrd45 at different stages. (A) At the bud stage, ankrd45 is expressed in the dorsal forerunner cells (arrowhead). (B) At the 10-somite stage, ankrd45 is expressed in the otic vesicle (OV), Kupffer’s vesicle (KV), and floor plate (FP). (C) ankrd45 is enriched in the otic vesicle, the pronephric duct (PD), and the floor plate at 24 hpf. (D) Real-time PCR showing the expression of ankrd45 at different stages or adult tissues as indicated. Bottom: bar graph showing the relative expression levels of ankrd45 at different stages or adult tissues as indicated. (E) Diagram showing the genomic structure of the zebrafish ankrd45 gene. Bottom shows the sequences of wild-type and mutant alleles (Δ5). Underlined sequence indicates the spCas9 target region, and the PAM sequence is indicated in red. (F–I’) Confocal images showing the cilia in the olfactory pit (F,F’), pronephric duct (G,G’), ear cristae (H,H’), and spinal cord (I,I’) in wild-type or mutant larvae visualized with anti-glycylated tubulin antibody. (J) Bar graph showing the length of cilia per arbitrary unit (a.u.) in different organs as indicated. OP, olfactory pit; EC, ear cristae; SC, spinal cord; NM, neuromast. Scale bars: 200 μm in A–C; 20 μm in F–I’. ns, not significant.

Expression Data

Gene:	ankrd45
Fish:	WT
Anatomical Terms:	floor plate forerunner cell group Kupffer's vesicle liver otic placode otic vesicle ovary pronephric duct testis whole organism
Stage Range:	Bud to Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ankrd45^{ouc2016/ouc2016}
Observed In:	cilium organization inner ear cilium neuromast cilium olfactory pit cilium spinal cord cilium
Stage:	Day 5

Phenotype Detail

Acknowledgments

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