ZFIN ID: ZDB-FIG-190723-8
Bremer et al., 2019 - The ubiquitin ligase PHR promotes directional regrowth of spinal zebrafish axons. Communications biology   2:195 Full text @ Commun Biol
ADDITIONAL FIGURES
EXPRESSION / LABELING:
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Fish:
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Anatomical Terms:
Stage Range: Day 5 to Days 7-13

Fig. 6

PHR does not control the morphology of MBP- and GFAP-positive glial cells. Time-lapse imaging over 9 h of regrowing Mauthner axons labeled by Tg(hspGFF62a) and Tg(UAS:gap431-20-RFP). Myelinating oligodendrocytes are transgenically labeled in Tg(MBP:EGFP-CAAX) in (aj) and GFAP-positive glial cells are labeled in TgBAC(GFAP:GFAP-GFP) in (kn). In wild type (ae) and phr mutant (fj), laser-mediated axon transection caused minor damage (fragmentation and rounding) of myelinating oligodendrocytes (white stars) around the transection site (yellow arrowheads), compared to the pretransection images (e, j). Except minor damage, myelinating oligodendrocytes neither displayed obvious morphological changes nor interfered with the transection site or formed any obvious scar tissue in any of the larvae (n = 4 wild type and n = 4 phr mutants). We did not observe any differences in the morphology of MBP-positive glial cells between wild type and phr mutant larvae before or after transection. Note also the rostrally extending axonal processes in wild type (retracting over time; shorter in (d) than in (c), marked by white arrows), and in the phr mutant (longer in (i) than in (h), marked by white arrows). kn Laser-mediated axon transection also caused minor damage to GFAP-positive glial cells in all larvae analyzed (n = 4 wild type, n = 4 phr mutants). Except minor damage, GFAP-positive glial cells neither displayed obvious morphological changes nor interfered with the transection site or formed any obvious scar tissue in any of the larvae (n = 4 wild type and n = 4 phr mutants). We did not observe any differences in the morphology of GFAP-positive glial cells between wild type and phr mutant axons before or after transection. Scale bar in (a) is 20 μm

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP nkhspGFF62ATg ; ue2Tg ; p411Tg ; mycbp2tn207b/tn207b transection: Mauthner neuron Day 5 to Days 7-13 oligodendrocyte IFL
ue2Tg ; nkhspGFF62ATg ; p411Tg transection: Mauthner neuron Day 5 to Days 7-13 oligodendrocyte IFL
GFP nkhspGFF62ATg ; p411Tg ; zf167Tg transection: Mauthner neuron Day 5 to Days 7-13 glial cell IFL
p411Tg ; mycbp2tn207b/tn207b ; nkhspGFF62ATg ; zf167Tg transection: Mauthner neuron Day 5 to Days 7-13 glial cell IFL
RFP nkhspGFF62ATg ; p411Tg ; zf167Tg transection: Mauthner neuron Day 5 to Days 7-13 Mauthner neuron IFL
Day 5 to Days 7-13 Mauthner neuron axon IFL
nkhspGFF62ATg ; ue2Tg ; p411Tg ; mycbp2tn207b/tn207b transection: Mauthner neuron Day 5 to Days 7-13 Mauthner neuron IFL
Day 5 to Days 7-13 Mauthner neuron axon IFL
p411Tg ; mycbp2tn207b/tn207b ; nkhspGFF62ATg ; zf167Tg transection: Mauthner neuron Day 5 to Days 7-13 Mauthner neuron IFL
Day 5 to Days 7-13 Mauthner neuron axon IFL
ue2Tg ; nkhspGFF62ATg ; p411Tg transection: Mauthner neuron Day 5 to Days 7-13 Mauthner neuron IFL
Day 5 to Days 7-13 Mauthner neuron axon IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Communications biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Commun Biol