ZFIN ID: ZDB-FIG-190723-5
Bremer et al., 2019 - The ubiquitin ligase PHR promotes directional regrowth of spinal zebrafish axons. Communications biology   2:195 Full text @ Commun Biol
Observed In:
Stage: Days 7-13

Fig. 4

Mutation in phr is causative for the regeneration defect in phr mutants. ac Mauthner axon at 48 hpt distal and proximal to the transection site in stitched, low magnification images of a wild-type phr+/+ axon (a), a heterozygous phrtn207b/+ axon (b) and a compound heterozygous phrtn207b/tp03 axon (c). Transection sites marked by a yellow arrowhead; rostrally directed projections in the phrtn207b/tp03 compound heterozygote are marked by white arrows (C). Scale bar for ac : 30 µm. Quantification of the extent of caudally directed regrowth in number of segments regrown caudally (d) and quantification of regrowth direction at the transection site (e), showing normal extent and directionality of regrowth in phr+/+ and phrtn207b/+ but reduced extent and misdirected regrowth in compound heterozygous phrtn207b/tp03. This shows that the regeneration deficits observed in phr mutants are indeed caused by a mutation in phr. N = 48 phr+/+, n = 53 heterozygous phrtn207b/+ and n = 13 compound heterozygous phrtn207b/tp03 were analyzed in (d, e). P-values were determined using two-tailed Student’s t-test (d), or Fisher exact test (e)

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
mycbp2tn207b/+ ; p201Tg ; nkhspGFF62ATg ; mycbp2tp203/+ transection: Mauthner neuron Days 7-13 Mauthner neuron axon extension involved in regeneration decreased efficacy, abnormal
Days 7-13 Mauthner neuron axon extension involved in regeneration process quality, abnormal
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