ZFIN ID: ZDB-FIG-190723-157
Hartwell et al., 2019 - Anteroposterior patterning of the zebrafish ear through Fgf- and Hh-dependent regulation of hmx3a expression. PLoS Genetics   15:e1008051 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: 14-19 somites to Prim-25
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: 14-19 somites to Prim-25

Fig 2 Expression of the otic anterior marker genes <italic>hmx3a</italic>, <italic>hmx2</italic> and <italic>pax5</italic> after early <italic>fgf3</italic> mis-expression.

In situ hybridisation of otic expression patterns in Tg(hsp70:fgf3) embryos following a 30-minute heat shock (HS) at the 10-somite stage (14 hpf). Controls (left-hand panels of each pair of images) were sibling non-transgenic embryos subjected to the same heat shock. Numbers in the dorsal view panels indicate the number of embryos with the phenotype shown and total number (e.g. 13/25) from a mixed batch of transgenic and non-transgenic embryos in each pair of panels; 50% of the batch was expected to be transgenic. (A–F) Two hours after heat shock (16 hpf), expression of hmx3a expanded to cover the entire otic region (B), but there was only a trace of expression of hmx2 or pax5 in the otic placode at this stage. Weak expression of pax5 in the hindbrain after heat shock (F) did not persist (L). (G–L) At 22.5 hpf (8.5 hours after HS), expression of all three genes had now expanded to cover the entire anteroposterior axis of the otic vesicle on the medial side. (M–R’) At 36 hpf (22 hours after HS), expression of hmx3a remained expanded across the otic anteroposterior axis (N,N’); expression of hmx2 was strong at the anterior and posterior poles, and weaker in central regions (P,P’), whereas expression of pax5 resolved into two discrete domains at the anterior and posterior poles of the otic vesicle, and was lost from central regions (R,R’). White arrowheads indicate regions that are normally free of expression in controls; black arrowheads mark ectopic expression in transgenic embryos. A–R are dorsal views showing both otic vesicles, with anterior to the top; M’–R’ are lateral views with anterior to the left. Scale bars, 50 μm (scale bar in A applies to A–L; in M applies to M–R; in M’ applies to M’–R’). For additional examples and time points for hmx2, see S4 Fig; for pax5, see S5 Fig.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
hmx2 AB heat shock 14-19 somites Not Detected otic vesicle anterior region ISH
26+ somites otic vesicle anterior region ISH
26+ somites Not Detected otic vesicle posterior region ISH
Prim-25 otic vesicle anterior region ISH
Prim-25 Not Detected otic vesicle posterior region ISH
mitfaw2/w2 ; zf115Tg heat shock 14-19 somites otic vesicle anterior region ISH
26+ somites otic vesicle anterior region ISH
26+ somites otic vesicle posterior region ISH
Prim-25 otic vesicle anterior region ISH
Prim-25 otic vesicle posterior region ISH
hmx3a AB heat shock 14-19 somites otic vesicle anterior region ISH
14-19 somites Not Detected otic vesicle posterior region ISH
26+ somites otic vesicle anterior region ISH
26+ somites Not Detected otic vesicle posterior region ISH
Prim-25 otic vesicle anterior region ISH
Prim-25 Not Detected otic vesicle posterior region ISH
mitfaw2/w2 ; zf115Tg heat shock 14-19 somites otic vesicle anterior region ISH
14-19 somites otic vesicle posterior region ISH
26+ somites otic vesicle anterior region ISH
26+ somites otic vesicle posterior region ISH
Prim-25 otic vesicle anterior region ISH
Prim-25 otic vesicle posterior region ISH
pax5 AB heat shock 14-19 somites Not Detected otic vesicle anterior region ISH
26+ somites otic vesicle anterior region ISH
26+ somites Not Detected otic vesicle posterior region ISH
Prim-25 otic vesicle anterior region ISH
Prim-25 Not Detected otic vesicle posterior region ISH
mitfaw2/w2 ; zf115Tg heat shock 14-19 somites otic vesicle anterior region ISH
26+ somites otic vesicle anterior region ISH
26+ somites otic vesicle posterior region ISH
Prim-25 otic vesicle anterior region ISH
Prim-25 otic vesicle posterior region ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
mitfaw2/w2 ; zf115Tg heat shock 14-19 somites inner ear morphogenesis decreased process quality, abnormal
14-19 somites inner ear posterior region anterioralized, abnormal
14-19 somites otic vesicle anterior region pax5 expression increased amount, abnormal
14-19 somites otic vesicle anterior region hmx2 expression increased amount, abnormal
14-19 somites otic vesicle posterior region hmx3a expression increased amount, abnormal
14-19 somites otic vesicle posterior region hmx3a expression mislocalised, abnormal
26+ somites inner ear morphogenesis decreased process quality, abnormal
26+ somites inner ear posterior region anterioralized, abnormal
26+ somites otic vesicle posterior region hmx2 expression increased amount, abnormal
26+ somites otic vesicle posterior region hmx3a expression increased amount, abnormal
26+ somites otic vesicle posterior region pax5 expression increased amount, abnormal
26+ somites otic vesicle posterior region hmx3a expression mislocalised, abnormal
26+ somites otic vesicle posterior region pax5 expression mislocalised, abnormal
26+ somites otic vesicle posterior region hmx2 expression mislocalised, abnormal
Prim-25 inner ear morphogenesis decreased process quality, abnormal
Prim-25 inner ear posterior region anterioralized, abnormal
Prim-25 otic vesicle posterior region pax5 expression increased amount, abnormal
Prim-25 otic vesicle posterior region hmx3a expression increased amount, abnormal
Prim-25 otic vesicle posterior region hmx2 expression increased amount, abnormal
Prim-25 otic vesicle posterior region pax5 expression mislocalised, abnormal
Prim-25 otic vesicle posterior region hmx3a expression mislocalised, abnormal
Prim-25 otic vesicle posterior region hmx2 expression mislocalised, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.