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Expression of endogenous ephb4a, dll4 and ephb2a in zebrafish after MOmediated smad1/5 knockdown. a-c. Expression of dll4, ephb2a and ephb4 as detected by whole-mount in situ hybridization after morpholino (MO)-mediated depletion of Smad1/5 at both 28hpf (a) and 36hpf (b). Graph (c) depicts observed expression of dll4 (blue), ephb2a (red) and ephb4a (green) in all embryos. At 28hpf, dll4 WT n=29 MO n=22, ephb2 WT n=25 MO n=23, ephb4a WT n=27 MO=31; at 36hpf dll4 WT n=31 MO ephb2a WT n=26 MO=23, ephb4a WT n=31 MO n=30). High expression levels are represented by solid colour, weak levels by pattern and absent expression by solid white. Zebrafish embryos shown are representative, values on the bottom right indicate number of embryos with the displayed phenotype per total number of embryos analysed, red bracket=dorsal aorta, white bracket=posterior cardinal and ventral vein(s).
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