ZFIN Figure: Pickett et al., 2018, Fig. 2

ZFIN ID: ZDB-FIG-190606-37

ZFIN ID: ZDB-FIG-190606-37

Pickett et al., 2018 - Cumulative mitochondrial activity correlates with ototoxin susceptibility in zebrafish mechanosensory hair cells. eLIFE 7 Full text @ Elife

ADDITIONAL FIGURES

PHENOTYPE:

Fish:	cdh23^tj264/tj264
Observed In:	neuromast hair cell mitochondrion neuromast hair cell regulation of mitochondrial membrane potential
Stage Range:	Day 5 to Days 7-13

Fig. 2

Acute mitochondrial activity is reduced in the absence of MET.

(A, B) Maximum projections of hair cells from WT/Het and sputnik mutant siblings incubated in JC-1 dye. Hair cells were imaged from a dorsal view, as indicated in the schematic shown in Figure 1—figure supplement 1B. (C) Mean JC-1 fluorescence plotted as a ratio of red:green. WT/Het: 0.25 ± 0.24 n = 8 fish; Mutant: 0.05 ± 0.07 n = 8 fish; mean ratio ± SD. Mann-Whitney U test was used to assess significance. Value for each fish represents the mean of 3 neuromasts. Scale bar = 5 μm.

Gene Expression Details No data available

Antibody Labeling Details No data available

Phenotype Details

Fish	Conditions	Stage	Phenotype
cdh23^tj264/tj264	control	Day 5 - Days 7-13	neuromast hair cell mitochondrion functionality, abnormal
		Day 5 - Days 7-13	neuromast hair cell regulation of mitochondrial membrane potential process quality, abnormal

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife