ZFIN ID: ZDB-FIG-190606-40
Pickett et al., 2018 - Cumulative mitochondrial activity correlates with ototoxin susceptibility in zebrafish mechanosensory hair cells. eLIFE   7 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Anatomical Term:
Stage: Day 5
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Day 5

Fig. 5

Mitochondrial activity depends on hair cell mechanotransduction.

(A, B) Maximum projections of hair cells from WT/Het and sputnik mutant siblings crossed to Tg[myo6b:mitoTimer]w208. (C) mitoTimer mean fluorescence ratio for WT/Het and sputnik larvae. WT/Het: 100 ± 18.7, n = 14 fish; Mutant: 34 ± 13.1,15 mutant fish; mean (% WT/Het)±SD. Value for each fish represents the mean of 2–4 neuromasts. (D, E) Maximum projections of mitoTimer-expressing hair cells from control larvae and larvae incubated in 200 μM benzamil. (F) mitoTimer mean fluorescence ratio for larvae incubated in benzamil compared to DMSO control. Control: 100 ± 28.7; Treated: 56.6 ± 22.7, n = 17 fish per group; mean (% Control)±SD. Value for each fish represents the mean of 2–4 neuromasts. Mann-Whitney U test was used to assess significance. All larvae imaged at 5dpf. Scale bar = 5 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed cdh23tj264/tj264; w208Tg control Day 5 neuromast hair cell mitochondrion IFL
w208Tg control Day 5 neuromast hair cell mitochondrion IFL
w208Tg chemical treatment: Benzamil Day 5 neuromast hair cell mitochondrion IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
cdh23tj264/tj264; w208Tg control Day 5 neuromast hair cell mitochondrion DsRed expression spatial pattern, abnormal
Day 5 neuromast hair cell mitochondrion functionality, abnormal
w208Tg chemical treatment: Benzamil Day 5 neuromast hair cell mitochondrion DsRed expression spatial pattern, abnormal
Day 5 neuromast hair cell mitochondrion functionality, abnormal
Acknowledgments:
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