Cumulative mitochondrial activity correlates with ototoxin susceptibility in zebrafish mechanosensory hair cells
- Pickett, S.B., Thomas, E.D., Sebe, J.Y., Linbo, T., Esterberg, R., Hailey, D.W., Raible, D.W.
- eLIFE 7: (Journal)
- Registered Authors
- Linbo, Tor, Raible, David
- cell biology, neuroscience, zebrafish
- MeSH Terms
- Anti-Bacterial Agents/toxicity*
- Cell Survival/drug effects
- Hair Cells, Vestibular/drug effects*
- Hair Cells, Vestibular/physiology*
- 30596476 Full text @ Elife
Pickett, S.B., Thomas, E.D., Sebe, J.Y., Linbo, T., Esterberg, R., Hailey, D.W., Raible, D.W. (2018) Cumulative mitochondrial activity correlates with ototoxin susceptibility in zebrafish mechanosensory hair cells. eLIFE. 7:.
Mitochondria play a prominent role in mechanosensory hair cell damage and death. Although hair cells are thought to be energetically demanding cells, how mitochondria respond to these demands and how this might relate to cell death is largely unexplored. Using genetically encoded indicators, we found mitochondrial calcium flux and oxidation are regulated by mechanotransduction and demonstrate that hair cell activity has both acute and long-term consequences on mitochondrial function. We tested whether variation in mitochondrial activity reflected differences in vulnerability of hair cells to the toxic drug neomycin. We observed that susceptibility did not correspond to the acute level of mitochondrial activity but rather to the cumulative history of that activity.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes