ZFIN ID: ZDB-FIG-190201-30
Joshi et al., 2018 - Mutation of a serine near the catalytic site of the choline acetyltransferase a gene almost completely abolishes motility of the zebrafish embryo. PLoS One   13:e0207747 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage: Long-pec

Fig. 1

Phenotype of chatatk64.

Wild-type embryo (A), chatatk64 mutant 3 hours after de-chorionation (B) and immediately after de-chorionation (C). Note that (B) is indistinguishable from (A). Birefringence is similar in wild-type (D) and chatatk64 mutant (E). Immunohistochemistry with phalloidin (red) marking F-actin, and α-actinin (green) (F, G), β-sarcoglycan (H, I) and α-bungarotoxin (J, K) do not reveal differences between wild-type (G, I, K) and chatatk64 mutant (F, H, J) at 48 hpf.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
chrna1 AB standard conditions Long-pec myotome acetylcholine-gated channel complex OTHR
Long-pec skeletal muscle cell acetylcholine-gated channel complex OTHR
chatatk64/tk64 (AB) standard conditions Long-pec myotome acetylcholine-gated channel complex OTHR
Long-pec skeletal muscle cell acetylcholine-gated channel complex OTHR
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-actn IHC AB standard conditions Long-pec myotome Z disc
IHC Long-pec skeletal muscle cell Z disc
IHC chatatk64/tk64 (AB) standard conditions Long-pec myotome Z disc
IHC Long-pec skeletal muscle cell Z disc
Phenotype Details
Fish Conditions Stage Phenotype
chatatk64/tk64 (AB) standard conditions Long-pec myotome myofibril assembly occurrence, normal
Long-pec myotome sarcomere organization occurrence, normal
Long-pec skeletal muscle cell sarcomere organized, normal
Long-pec skeletal muscle cell skeletal muscle myofibril organized, normal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One