ZFIN ID: ZDB-FIG-180927-27
Xiao et al., 2018 - The roles of SMYD4 in epigenetic regulation of cardiac development in zebrafish. PLoS Genetics   14:e1007578 Full text @ PLoS Genet.
Observed In:
Stage Range: Long-pec to Day 4

Fig. 3

Phenotypical analyses of MZsmyd4L544Efs*1 embryos.

(A) Representative images of the MZsmyd4L544Efs*1 and control embryos at 48 hpf, exhibiting congested blood flow in the ventral vein; (B) Representative images of the MZsmyd4L544Efs*1 and control embryos at 96 hpf, displaying severe pericardial edema; (C) Representative images of the MZsmyd4L544Efs*1 and control hearts at 72 hpf, demonstrating abnormal cardiac morphology and left-right looping defects; (D) Significantly more MZsmyd4L544Efs*1 embryos had abnormal cardiac development than control fish Tg (cmcl2:GFP) (61% ± 4.0% mutants vs. 14% ± 4.6% controls, n = 100, p < 0.01**); (E) Comparison of left-right patterning defects in the MZsmyd4L544Efs*1 (n = 100) and control (n = 100) embryos (green: normal L-loop; blue: straight; dark blue: D-loop). Only 10% of the control embryos showed an abnormal “straight” cardiac left-right asymmetry pattern. However, 48% of MZsmyd4L544Efs*1 embryos exhibited a “straight” left-right asymmetry pattern, and 11% showed a “D-loop” left-right asymmetry pattern; (F) Representative confocal images of the ventricular wall and 3D re-construction images of the MZsmyd4L544Efs*1 and control hearts at 96 hpf. The confocal scanned images demonstrated hypoplastic wall with significant reduction of trabeculation in MZsmyd4L544Efs*1 ventricle when compared to the wild-type control ventricle. The 3D-reconstruction of the hearts confirmed left-right looping defects in MZsmyd4L544Efs*1 hearts (For a better view of 3D-structures, see supplemental material S1 and S2 Movies). (G) Histological sections and H&E staining images of the MZsmyd4L544Efs*1 and control hearts at 96 hpf, demonstrating abnormal ventricular walls and reduced trabeculation; (H) The quantitative analysis of the number of cardiomyocytes in the ventricle displayed that MZsmyd4 L544Efs*1 had significantly reduced cardiomyocytes (p<0.01, **).

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
smyd4zf3020/zf3020; zf2036Tg standard conditions Long-pec blood accumulation ventral aorta, abnormal
Protruding-mouth determination of heart left/right asymmetry disrupted, abnormal
Protruding-mouth heart looping disrupted, abnormal
Day 4 cardiac ventricle cardiac muscle cell decreased amount, abnormal
Day 4 cardiac ventricle hypoplastic, abnormal
Day 4 heart trabecula morphogenesis disrupted, abnormal
Day 4 pericardium edematous, abnormal
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.