Fig. 7

Spatial expression of the caspase-8 family members casp8a, casp10 and casp20 at 24, 48, 72 and 96 hpf.

Lateral views of whole embryos stained for casp8a (A-D), casp10 (E-H) and casp20 (I-L) by ISH. Casp8a is expressed in the hindbrain (hb), muscles (ms) and at the floorplate (fp) at 24 hpf (A). Its expression is then strongly detected in the retina (r), diencephalon (di), midbrain (mb), hindbrain and muscles (ms) at 48 hpf (B). Additional expression in the intestinal bulb (ib) is observed at 72 and 96 hpf (C, D). Casp10 expression is detected at the floorplate at 24 and 48 hpf (E, F) and in the pharyngeal arches (pa) at 48 hpf (F). A strong expression is detected in the pharyngeal arches, muscles and intestinal bulb at 72 hpf (G). Casp10 remains highly detected in the pharyngeal arches and intestinal bulb at 96 hpf (H). Casp20 is strongly expressed in the nervous system (ns), vascular system (vs), proctodeum (pr) and throughout the gut (g) at 24 hpf (I). Its expression becomes restricted to the pharyngeal arches and the intestinal bulb at 48, 72 and 96 hpf (J-L). Scale bar: 200 μm.